双歧杆菌联合干扰素对病毒性腹泻患儿中性粒细胞及心肌酶谱的影响

Effects of bifidobacterium combined with interferon on neutrophil and myocardial zymogram in children patients with viral diarrhea

目的 探讨双歧杆菌三联活菌联合注射用重组人干扰素α-1b对病毒性腹泻患儿中性粒细胞杆状核、分叶核及血清心肌酶谱水平的影响。方法 回顾性收集2017年4月至2019年12月该院93例病毒性腹泻患儿作为研究对象,根据治疗方案分为两组,除常规治疗外,对照组(n=46)予以双歧杆菌三联活菌治疗,观察组(n=47)在此基础上肌内注射重组人干扰素α-1b。对比两组临床疗效、症状缓解时间及治疗前后中性粒细胞杆状核、分叶核、血清炎性细胞因子[白细胞介素(IL)-6、IL-8、肿瘤坏死因子-α(TNF-α)]、心肌酶谱[肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、羟丁酸脱氢酶(HBDH)、乳酸脱氢酶(LDH)]及免疫功能水平。结果 观察组治疗总有效率95.74%(45/47)高于对照组80.43%(37/46),差异有统计学意义(P<0.05)。观察组的止呕、止泻及退热时间均短于对照组,差异有统计学意义(P<0.05)。治疗1、3 d后观察组中性粒细胞杆状核、分叶核计数较对照组低,差异有统计学意义(P<0.05)。治疗1、3 d后观察组血清炎性因子IL-6、IL-8及TNF-α水平低于对照组,差异有统计学意义(P<0.05)。治疗1、3 d后观察组血清CK、CK-MB、LDH及HBDH水平低于对照组,差异有统计学意义(P<0.05)。治疗1、3 d后观察组CD4^(+)、CD4^(+)/CD8^(+)水平高于对照组,CD8^(+)水平低于对照组,差异有统计学意义(P<0.05)。结论 双歧杆菌三联活菌联合重组人干扰素α-1b通过降低心肌酶谱水平,调节免疫炎性反应,有助于增强治疗效果,缩短病毒性腹泻患儿症状改善时间。

Objective To investigate the effects of bifidobacterium triple viable bacteria combined with recombinant human interferon α-1 b injection on neutrophil rod nucleus, lobular nucleus and serum myocardial zymogram in children patients with viral diarrhea.Methods Ninety-three children patients with viral diarrhea in this hospital from April 2017 to December 2019 were retrospectively collected as the study subjects and divided into two groups according to the treatment regimen.The two groups were given the routine treatment.On this basis, 46 cases treated with bifidobacterium triple viable bacteria served as the control group, and 47 cases treated by bifidobacterium triple viable bacteria combined with recombinant human interferon α-1 b injection as the observation group.The clinical efficacy, symptom relief time, neutrophil rod nucleus, lobular nucleus, serum inflammatory cytokines(IL-6,IL-8,TNF-α),myocardial zymogram [creatine kinase(CK),creatine kinase isoenzyme(CK-MB),hydroxybutyrate dehydrogenase(HBDH),lactate dehydrogenase(LDH)] and immune function before and after treatment were compared between the two groups.Results The total effective rate in the observation group was 95.74%(45/47),which was higher than 80.43%(37/46) in the control group, and the difference was statistically significant(P<0.05).The antiemetic, antidiarrheal and antipyretic time of the observation group was shorter than that of the control group(P<0.05).The count of neutrophil rod nucleus and lobulated nucleus after 1,3 d treatment in the observation group was lower than that in the control group, and the differences were statistically significant(P<0.05).The serum inflammatory factors IL-6,IL-8 and TNF-α levels after 1,3 d treatment in the observation group was lower than those in the control group(P<0.05).Serum CK,CK-MB,LDH and HBDH levels after 1,3 d treatment in the observation group were lower than those in the control group, and the differences were statistically significant(P<0.05).The CD4^(+)and CD4^(+)/CD8^(+)levels after 1,3 d treatment in the observation group were higher than those in the control group, the CD8^(+)level was lower than that in the control group, and the differences were statistically significant(P<0.05).Conclusion Bifidobacterium triple viable bacteria combined with recombinant human interferon α-1 b is conducive to enhance the treatment effect and shorten the symptom improvement time in the children patients with viral diarrhea by reducing the level of myocardial zymogram and regulating immune inflammatory response.