去甲斑蝥素对鼻咽癌CNE-2细胞增殖和凋亡的影响

Effects of norcantharidin on proliferation and apoptosis of nasopharyngeal carcinoma CNE-2 cells

目的研究去甲斑蝥素(norcantharidin,NCTD)对鼻咽癌CNE-2细胞增殖和凋亡的影响。方法CNE-2细胞分为对照组和不同剂量NCTD组,分别用含有0、35、70、140μmol/L NCTD的DMEM高糖培养基培养。采用CCK-8法、克隆形成实验、流式细胞术分别检测CNE-2细胞的增殖速率、克隆形成能力和凋亡率。实时定量PCR法检测各组CNE-2细胞中Cyclin B的mRNA表达水平。结果48 h时,NCTD对CNE-2细胞的半数抑制浓度(IC_(50))值为(89.07±3.702)μmol/L;与对照组比较,35、70、140μmol/L NCTD组细胞增殖、克隆能力均下降,差异有统计学意义(P<0.05),细胞周期阻滞在G2/M期,细胞中Cyclin B的表达水平降低,差异有统计学意义(P<0.05),细胞凋亡率升高,差异有统计学意义(P<0.05)。结论NCTD能够诱导CNE-2细胞的凋亡并通过下调Cyclin B的表达抑制细胞的增殖和克隆能力。

Objective:To study the effects of norcantharidin(NCTD)on proliferation and apoptosis of nasopharyngeal carcinoma(NPC)CNE-2 cells.Methods:CNE-2 cells were divided into control group and different doses of NCTD group,and cultured in DMEM high glucose medium containing 0,35,70,140μmol/L NCTD.The proliferation rate,clonogenesis ability and apoptosis rate of CNE-2 cells were determined by CCK-8 assay,clonogenesis assay and flow cytometry,respectively.Cyclin B mRNA expression level in CNE-2 cells was detected by real-time quantitative PCR.Results:At 48h,the IC_(50) value of NCTD on CNE-2 cells was(89.07±3.702)μmol/L.Compared with the control group,35,70 and 140μmol/L NCTD groups significantly decreased cell proliferation and clonal formation ability(P<0.05),cell cycle arrest in G2/M phase,Cyclin B expression significantly decreased(P<0.05),and the apoptosis rate was significantly increased((P<0.05).Conclusion:NCTD can induce the apoptosis of CNE-2 cells and inhibit the proliferation and clonogenesis of CNE-2 cells by down-regulating Cyclin B expression.