恶性黑色素瘤组织中miRNA-186和miRNA-146a表达及与病理特征和预后的关系研究

Expression of miRNA-186 and miRNA-146a in malignant melanoma and their relationship with pathological features and prognosis

目的探讨恶性黑色素瘤组织中微小RNA(miRNA)-186和miRNA-146a表达水平及与病理特征和预后的关系。方法收集2017年1月—2021年1月浙江金华广福肿瘤医院行手术切除的93例恶性黑色素瘤患者肿瘤组织及瘤旁组织,采用实时荧光定量PCR(qRT-PCR)法测定miRNA-186和miRNA-146a表达。所有患者均随访至2022年1月,评估患者预后情况。比较肿瘤组织与瘤旁组织中miRNA-186和miRNA-146a相对表达量;比较不同病理特征肿瘤组织中miRNA-186和miRNA-146a相对表达量;分析miRNA-186和miRNA-146a表达水平与预后的关系;采用多因素logistic回归分析影响恶性黑色素瘤患者预后的危险因素。结果肿瘤组织中miRNA-186相对表达量高于瘤旁组织,miRNA-146a低于瘤旁组织(均P<0.05)。不同性别、年龄、肿瘤部位和Clark分级患者miRNA-186和miRNA-146a相对表达量比较,差异均无统计学意义(均P>0.05);IV期患者miRNA-186相对表达量高于II期和III期患者,淋巴结转移患者miRNA-186相对表达量高于无淋巴结转移患者(均P<0.05);IV期患者miRNA-146a相对表达量低于II期和III期患者,淋巴结转移患者miRNA-146a相对表达量低于无淋巴结转移患者(均P<0.05)。随访至2022年1月,93例患者中,生存74例,死亡19例。死亡组患者肿瘤组织miRNA-186相对表达量高于生存组,miRNA-146a相对表达量低于生存组(均P<0.05)。多因素logistic回归分析显示,临床分期、淋巴结转移、miRNA-186表达和miRNA-146a表达是影响恶性黑色素瘤患者预后的危险因素。结论恶性黑色素瘤组织中miRNA-186高表达、miRNA-146a低表达,miRNA-186和miRNA-146a表达水平与临床分期、淋巴结转移和患者预后密切相关。

Objective To investigate the expression levels of miRNA-186 and miRNA-146a in malignant melanoma and their relationship with pathological features and prognosis.Methods Ninety three samples of cancer tissue and pericancerous tissue were collected from patients with malignant melanoma treated in Zhejiang Jinhua Guangfu Cancer Hospital from January 2017 to January 2021.The expression of miRNA-186 and miRNA-146a was detected by quantitative real-time PCR(qRT-PCR).All patients were followed up to January 2022 and the prognosis of the patients was assessed.The relative expression of miRNA-186 and miRNA-146a in cancer tissues and adjacent tissues were compared.The association of miRNA-186 and miRNA-146a expression with pathological characteristics and prognosis of malignant melanoma patients was analyzed.Multivariate logistic regression was used to analyze the risk factors affecting the prognosis of patients with malignant melanoma.Results The relative expression of miRNA-186 in cancer tissues was higher and expression of miRNA-146a was lower in cancer tissues was lower than those in adjacent tissues(P<0.05).The relative expression of miRNA-186 and miRNA-146a was not associated with gender,age,tumor site and Clark grade(all P>0.05).The relative expression of miRNA-186 in stage IV cancer was higher than that in stage II and III,and relative expression of miRNA-186 in tumors with lymph node metastasis was higher than that without lymph node metastasis(all P<0.05).The relative expression of miRNA-146a in stage IV was lower than that in stage II and III,and the relative expression of miRNA-146a in tumors with lymph node metastasis was lower than that without lymph node metastasis(all P<0.05).During the follow-up period among 93 patients,74 survived and 19 died.The relative expression of miRNA-186 in death group was higher than that in survival group,while the relative expression of miRNA-146a in death group was lower than that in survival group(all P<0.05).Multivariate logistic regression analysis showed that clinical stage,lymph node metastasis,miRNA-186 expression and miRNA-146a expression were independent risk factors affecting the prognosis of patients with malignant melanoma.Conclusion The expression levels of miRNA-186 and miRNA-146a are closely related to clinical stage,lymph node metastasis and prognosis of patients.