芦荟大黄素-锌(Ⅱ)配合物的合成、表征及抗癌活性研究

Study on the synthesis,characterization and anticancer activity of aloe emodin-zinc(Ⅱ) complex

目的:合成芦荟大黄素-锌(Ⅱ)配合物,对其进行结构表征分析,并比较芦荟大黄素(AE)与Zn^(2+)配位前后的抗癌活性变化。方法:以芦荟大黄素和醋酸锌为原料在无水乙醇中合成芦荟大黄素-Zn(Ⅱ)配合物,通过UV-vis、FT-IR、H1 NMR、TG-DTA、配位滴定法表征其结构,采用MTT法比较芦荟大黄素与Zn^(2+)配位前后的抗癌活性变化。结果:合成了芦荟大黄素-Zn(Ⅱ)配合物,确定了其可能的结构式,MTT法结果显示相比于配体,芦荟大黄素-Zn(Ⅱ)配合物对HepG2细胞生长抑制率平均提高了5.95%、半数抑制浓度降低了30.82%。结论:芦荟大黄素与Zn^(2+)进行络合后,增强了配体本身的抗癌活性。

Objective: To synthesize aloe emodin-zinc (Zn) (Ⅱ) complex and characterize its structure, and compare the anticancer activity of aloe emodin before and after the coordination with Zn^(2+). Method: Aloe emodin-Zn (Ⅱ) complex was synthesized using aloe emodin and zinc acetate as raw materials in anhydrous ethanol. Its structure was characterized by UV-vis,FT-IR, H1 NMR, TG-DTA and coordination titration, and the anticancer activity of aloe emodin before and after the coordination with Zn^(2+) were compared by MTT method. Result: Aloe emodin-Zn (Ⅱ) complex was synthesized and its possible structural formula was determined. MTT assay results showed that compared with the ligand, the growth inhibition rate of aloe emodin-Zn(Ⅱ) complex on HepG2 cells was increased by 5.95% on average, and the half inhibition concentration was decreased by 30.82%.Conclusion: Aloe emodin complexed with Zn^(2+) can enhance the anticancer activity of the ligand itself.