两种方法提取琉璃苣叶多糖的理化性质及生理活性

Physicochemical properties and physiological activities of polysaccharides extracted from Borage leaves by two methods

分别采用纤维素酶辅助和微波辅助两种方法提取琉璃苣叶多糖,得到的多糖分别记为BLP-1和BLP-2。采用高效凝胶渗透色谱仪、FTIR、SEM及TGA对BLP-1和BLP-2进行了表征,通过α-葡萄糖苷酶和α-淀粉酶的抑制效果评价样品的体外降血糖活性,通过检测对人肝癌细胞Hep G2、人乳腺癌细胞MX-1和人肺腺癌细胞A549的抑制率评价了样品的抗癌活性,通过检测小鼠巨噬细胞RAW246.7的免疫因子分泌评价样品免疫活性。结果表明,BLP-1和BLP-2具有不同的多糖和蛋白质质量分数。BLP-1和BLP-2由L-阿拉伯糖、D-半乳糖、葡萄糖、木糖组成,但摩尔分数不同,其相对分子质量分别为20103和22652。BLP-2比BLP-1具有更稳定的结构。这两种多糖具有抑制α-葡萄糖苷酶和α-淀粉酶活性、抑制上述3种癌细胞增殖以及激活小鼠巨噬细胞RAW264.7细胞分泌免疫细胞因子以介导细胞免疫反应的潜力。

Borage leave polysaccharides,obtained by cellulose enzyme assisted extraction and microwave assisted extraction,were named as BLP-1 and BLP-2,respectively,and then characterized by high performance gel permeation chromatography,FTIR,SEM and TGA.Furthermore,the in vitro hypoglycemic,anti-cancer and immune activities of BLP-1 and BLP-2 was evaluated by the inhibitory effect ofα-glucosidase andα-amylase,inhibition rate of HepG2 human hepatoma cells,MX-1 human breast cancer cells and A549 human lung adenocarcinoma cells,and immune factor secretion detection in mouse macrophages RAW246.7,respectively.The results showed that the sugar and protein contents in BLP-1 and BLP-2 were different.Though both of BLP-1 and BLP-2 were composed of L-arabinose,D-galactose,glucose,and xylose,their molar fraction was different with corresponding relative molecular mass of 20103and 22652,respectively.Meanwhile,BLP-2 exhibited a more stable structure than BLP-1.The two polysaccharides showed potential in inhibitingα-glucosidase andα-amylase activities,suppressing the above cancer cells proliferation,and activating mouse macrophages RAW264.7 cells to secret immune cytokines for cellular immune response mediation.