Raf-1蛋白激酶及VEGF在大肠腺癌中的表达及临床意义研究

Study on the Expression and Clinical Significance of Raf-1 Protein Kinase and VEGF in Colorectal Adenocarcinoma

目的探究Raf-1蛋白激酶、血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)在大肠腺癌中表达情况及临床意义。方法选取江苏省东台市人民医院2021年1—12月期间收治的60例大肠腺癌患者为研究对象,均接受手术切除治疗,收集其病灶样本,以免疫组化法检测Raf-1蛋白激酶、VEGF阳性检出率,比较两种指标单独、联合阳性检出率,并比较两种指标在不同肿瘤特征(不同肿瘤直径、不同分化程度、是否有淋巴结转移、浸润深度、Dukes分期)中阳性检出率。结果Raf-1蛋白激酶联合VEGF对病灶阳性检出率为60.00%,较其单独诊断高(28.33%、41.67%),差异有统计学意义(P<0.05);VEGF在有淋巴结转移、浸润深度突破浆膜层、Dukes分期为C期+D期中阳性检出率较无淋巴结转移、浸润深度未突破浆膜、Dukes分期为A期+B期高,差异有统计学意义(χ^(2)=7.143、6.094、5.503,P=0.008、0.014、0.019);VEGF在不同肿瘤直径、肿瘤分化程度中阳性检出率比较,差异无统计学意义(χ^(2)=0.939、0.471,P=0.333、0.493);Raf-1蛋白激酶在不同肿瘤直径、分化程度、有无淋巴结转移、浸润深度、Dukes分期中阳性检出率比较,差异无统计学意义(χ^(2)=0.134、0.398、3.502、0.764、0.396,P=0.714、0.528、0.061、0.328、0.529)。结论VEGF阳性检出率提升,提示存在淋巴结转移、浸润深度增加、Dukes分期增加,可用于诊断及预后评估,且两种指标联合应用可提升阳性检出率。

Objective To investigate the expression of Raf-1 protein kinase and vascular endothelial growth factor(VEGF)in colorectal adenocarcinoma and their clinical significance.Methods 60 patients with colorectal adenocarcinoma who were admitted to Dongtai People’s Hospital of Jiangsu Province during January to December 2021 were selected for the study.All of them were treated with surgical resection,and their lesion samples were collected to detect the positive detection rate of Raf-1 protein kinase and VEGF by immunohistochemistry.The detection rates of the two indicators alone and in combination were compared.The positive detection rates of the two indicators were also compared in different tumor characteristics(different tumor diameters,different degrees of differentiation,presence or absence of lymph node metastasis,depth of infiltration,and Dukes stage).Results The positive detection rate of Raf-1protein kinase in combination with VEGF was 60.00%,which was higher than that of VEGF alone(28.33%,41.67%),and the difference was statistically significant(P<0.05).The positive detection rate of VEGF was higher in the presence of lymph node metastasis,infiltration depth not breaking through the plasma membrane,and Dukes stage A+B than in the absence of lymph node metastasis.The differences were statistically significant(χ^(2)=7.143,6.094,5.503,P=0.008,0.014,0.019).The positive detection rate of VEGF was compared in different tumor diameters and tumor differentiation degrees.The difference was not statistically significant(χ^(2)=0.939,0.471,P=0.333,0.493).The positive detection rate of Raf-1 protein kinase in different tumor diameters,differentiation,presence or absence of lymph node metastasis,depth of infiltration and Dukes stage was compared,and the differences were not statistically significant(χ^(2)=0.134,0.398,3.502,0.764,0.396,P=0.714,0.528,0.061,0.328,0.529).Conclusion The increased positive detection rate of VEGF suggests the presence of lymph node metastasis,increased depth of infiltration,and increased Dukes’stage,which can be used for diagnosis and prognostic assessment,and the combined application of the two indicators can improve the positive detection rate.