利用微计算机断层扫描技术重建褐飞虱内部结构

Reconstruction of internal structures of Nilaparvata lugens using micro-computer tomography technology (Micro CT)

褐飞虱(Nilaparvata lugens)是一种重要的水稻害虫。本研究利用微计算机断层扫描技术(micro-computer tomography technology, Micro CT)获得了褐飞虱成虫的断层扫描图,通过人工建模和深度学习相结合的方式对褐飞虱内部组织和器官进行三维建模,获得了褐飞虱中枢神经系统、肌肉组织、消化道和生殖系统的三维模型,保留了这些结构的原始形态,准确地还原了褐飞虱体内各组织和器官的空间排布,并利用Dragonfly软件对昆虫的内部组织进行了空间体积的测量分析。本研究建立并完善了昆虫组织和器官的三维建模技术,有助于更为精准地观察昆虫的内部组织结构,可用于昆虫形态和器官发育的表型观察,为昆虫发育和基因功能研究提供了新技术和新方法。

The brown planthopper(Nilaparvata lugens) is an important rice pest. In this study, the tomographic images of adult brown planthopper were obtained by using micro-computer tomography technology(Micro CT).Three-dimensional models of internal tissues and organs of the brown planthopper were established by combining manual modeling and deep learning. Three-dimensional models of the central nervous system, muscle tissue,alimentary canal and reproductive system of the brown planthopper were obtained. These models preserved the original morphologies of these structures and accurately restored the spatial arrangement of all tissues and organs within the brown planthopper. Measurements of the internal organization of insects were analyzed using Dragonfly software. This study established and refined the three-dimensional reconstruction technique of insect tissues and organs, which can contribute to a more precise view of the internal organization structure of insects and can be used for phenotypic observation of insect morphology and organ development. It provides a new technique for insect development investigation and gene function analysis in entomology.