贝母属植物叶绿体基因组结构与系统发育的关系

Relationship between Chloroplast Genomes Structures from Fritillaria Plants and Phylogeny

贝母属植物形态相似,难以区分。通过对叶绿体基因组进行分析,可以为贝母属植物的系统发育、分子鉴定和开发利用提供理论依据。我们基于已发表的贝母属物种的叶绿体基因组序列,利用相关生物信息学方法对其叶绿体基因组进行分析。结果显示,贝母属29个物种叶绿体基因组大小在151058~152434 bp。基因数量为128~133个,r RNA编码基因数目稳定,蛋白质编码基因和t RNA编码基因存在差异。四分区分析发现,IRb/SSC边界存在明显差异。串联重复和单核苷酸重复含量最丰富。共线性分析未检测到基因重排,其叶绿体基因组高度保守。基于叶绿体基因组的系统发育分析表明,贝母属植物分为4个主要分支。对贝母叶绿体基因组进行核苷酸多态性分析,发现IR比单拷贝区更保守,非编码区的突变频率比编码区高。

It is difficult to distinguish between plants of the Fritillaria because of their morphological similarity.Characteristics and phylogeny of chloroplast genomes,can provide a theoretical basis for study of the phylogeny,molecular identification,and resource exploitation of the Fritillaria.Based on the published chloroplast genome sequences of 29 Fritillaria species,their chloroplast genomes were analyzed using bioinformatics methods.The results showed that,the chloroplast genome is between 151058 bp and 152434 bp.There are 128 to 133 genes in the Fritillaria,with a stable number of rRNA-coding genes,and differences in protein-encoding and tRNA-encoding genes.Significant differences exist at the IRb/SSC boundary.The highest content of tandem repeats and mononucleotide.No gene rearrangements were detected by covariance analysis,reflecting the high degree of conservation in the chloroplast genome of the Fritillaria.Phylogenetic analysis based on the chloroplast genome indicates that the Fritillaria can be divided into four major phylogenetic clades.Nucleotide polymorphism analysis of the chloroplast genome of Fritillaria found that the IR was more conserved than the single-copy regions,and the non-coding region was mutated more frequently than the coding region.