百合“索邦”病程相关蛋白基因LhSorPR10-2的克隆与分析

Cloning and Analysis of Pathogenesis-related Protein Gene LhSorPR10-2 from‘Sorbonne’Lily

病程相关蛋白(Pathogenesis-related Proteins,PR)是植物在受病原物侵染过程中诱导产生的一类蛋白.为研究百合“索邦”PR10基因的生物学功能,本研究在灰霉菌(Botrytis elliptica)处理不同时间的百合“索邦”叶片转录组数据库中筛选获得显著诱导表达的一个病程相关蛋白PR10基因,命名为“LhSorPR10-2”,并对其进行生物信息学分析、表达模式分析及其启动子克隆分析.结果显示:LhSorPR10-2全长761 bp,开放阅读框为474 bp,编码158个氨基酸,等电点为5.72.LhSorPR10-2在百合“索邦”中的表达具有组织特异性,在叶中表达量最高;该基因可被病原菌椭圆葡萄孢(Botrytis elliptica)和尖孢镰刀菌(Fusariumoxy sporum)诱导上调表达,并能被植物激素茉莉酸甲酯(MeJA)、水杨酸(SA)和乙烯利(ETH)诱导响应以及高温(50℃)和低温(0℃)胁迫诱导表达.另外,启动子区的顺式作用元件分析显示LhSorPR10-2启动子区含有大量与光信号、植物激素、胁迫和分生组织等相关的功能域,推测其可能在生物及非生物胁迫响应过程中发挥重要作用.本研究旨在了解百合“索邦”PR10基因的有关特性及在抗病防御反应中的作用,为今后揭示该基因的抗性分子机制提供理论基础.

Pathogenesis-related proteins(PR)are a class of proteins induced by pathogens in plants.In order to study the biological function of PR10 gene,a pathogenesis-related protein PR10 gene named‘LhSorPR10-2’was screened from the transcriptome database of‘Sorbonne’lily leaves treated with Botrytis elliptica for different period of time.Bioinformatics analysis,expression pattern analysis and promoter analysis were carried out.The results showed that the total length of LhSorPR10-2 was 761 bp with a 474 bp open reading frame,encoding 158 amino acids,and the isoelectric point was 5.72.The expression of LhSorPR10-2 in‘Sorbonne’lily was tissue-specific,which had the highest expression in leaves.The expression of this gene could be induced by Botrytis elliptica and Fusariumoxy sporum,also by phytohormones such as methyl jasmonate(MeJA),salicylic acid(SA)and ethephon(ETH)and by temperature stresses such as high temperature(50℃)and low temperature(0℃).In addition,cis-acting element analysis of the promoter region showed that the promoter region of LhSorPR10-2 contained a large number of functional domains related to light signals,phytohormones,stress and meristems,which may play an important role in the process of biotic and abiotic stress response.The aim of this study was to understand the characteristics of PR10 gene in‘Sorbonne’lily and its role in disease resistance and defense response,and to provide a theoretical basis for revealing the molecular mechanism of stress tolerance in the future.