BBD法提取芦荟苷工艺的优化及稳定性分析

BBD Method for Optimization and Stability Analysis of Aloin

通过Box-Behnken Design效应面法(BBD)优化超声波醇提法提取芦荟皮中芦荟苷的工艺条件;并通过单因素试验对芦荟苷进行初步稳定性分析。以提取时间、超声功率、料液比、乙醇体积分数作为影响芦荟苷提取的单因素进行试验,建立四因素三水平的响应面数据模型,获得最佳提取率的工艺为:提取时间为10 min,功率为700 W,液料比为1∶50(g/mL),乙醇体积分数为64%,此时芦荟苷的产率为7.215 mg/g;经稳定性初步研究表明,芦荟苷适宜保存在低温避光条件下,溶液状态则还需加入抗坏血酸或柠檬酸,调节pH=5。

Box-behnken design-response surface method(BBD)was used to optimize the extraction conditions of aloin from aloe vera skin by ultrasonic alcohol extraction in order to improve the yield of aloin.The stability of aloin was preliminarily analyzed by single factor test.Extraction time,ultrasonic power,solid-liquid ratio and ethanol concentration were used as the single factors affecting the extraction of aloin.A response surface data model with four factors and three levels was established.The optimal extraction rate was obtained as follows:extraction time of 10 min,power of 700 W,liquid-solid ratio of 1∶50(g/mL),ethanol concentration 64%,the yield of aloin was 7.215 mg/g.According to the preliminary stability study,the related products are suitable to be stored at low temperature and protected from light.If they are in solution state,in ascorbic acid or citric acid need to be added(pH=5).