响应面法优化铁皮石斛叶发酵饮料工艺

Optimization of Fermented Beverage from Leaves of Dendrobium Officinale by Response Surface Methodology

采用植物乳杆菌发酵铁皮石斛叶制备饮料。以低聚异麦芽糖作为碳源,在单因素实验基础上,以饮料的感官评分作为评价指标,采用响应面法优化最佳的发酵工艺。结果表明:最佳的发酵工艺为植物乳杆菌添加量0.1g/L、发酵温度37℃、发酵时间为36h,所得发酵饮料感官评分为88分,粗多糖为20g/L,产品颜色呈浅黄色,口感清爽,酸甜适宜。

Beverage was prepared from Dendrobium candidum leaves fermented by Lactobacillus plantarum.Taking the sensory score of the beverage as the evaluation index.The isomalto oligosaccharide as the carbon source.The optimal fermentation process of Dendrobium candidum leaves were optimized by response surface methodology.The results showed that the optimum conditions were as follows:the addition amount of Lactobacillus plantarum was 0.1g/L,the fermentation temperature was 37℃,the fermentation time was 36h,the sensory score of the fermented Dendrobium candidum leaves beverage was 88 points,and the crude polysaccharide was 20g/L.The color of the product was light yellow,and the taste was fresh and suitable for sour and sweet.