摘要
目的 分析卵巢癌和卵巢良性肿瘤患者血清Th1型细胞因子IFN-γ和Th2型细胞因子IL-4水平及病灶黏蛋白-1糖蛋白(MUC1)和病灶组织中乳腺癌易感基因相关蛋白1(BAP1)表达的差异。方法 选取2015年至2022年青岛市妇女儿童医院收治的60例卵巢癌患者,另选同期手术治疗且经病理确诊的50例卵巢良性肿瘤患者,再选同期体检的40例健康妇女为研究对象。酶联免疫吸附法检测IFN-γ和IL-4的水平,免疫组织化学法检测病灶组织中MUC1和BAP1表达;ROC曲线分析IFN-γ和IL-4对卵巢癌诊断价值,收集术后病理组织,检测MUC1和BAP1阳性表达情况并分析其与病理特征之间的关系,采用Spearman法分析其与肿瘤恶性程度关系。结果 卵巢癌组患者血清IFN-γ水平低于卵巢良性肿瘤组与健康组,IL-4水平高于卵巢良性肿瘤组与健康组(P<0.05);卵巢良性肿瘤组与健康组IFN-γ和IL-4水平无统计学意义(P>0.05)。ROC曲线显示,IFN-γ和IL-4的AUC分别为0.748和0.796,灵敏度分别为98.2%和80.9%,特异度分别为40.0%和62.5%。卵巢癌组织中MUC1和BAP1阳性表达率高于卵巢良性肿瘤(P<0.05)。MUC1和BAP1阳性表达在年龄、肿瘤直径方面无统计学意义(P>0.05),在FIGO分期、病理分级、淋巴结转移方面,差异具有统计学意义(P<0.05)。Spearman法分析显示,MUC1和BAP1表达与FIGO分期、病理分级和淋巴结转移呈正相关(P<0.05)。结论 血清中IFN-γ和IL-4水平及病灶组织中的MUC1和BAP1的阳性程度或可作为卵巢癌的辅助诊断指标。
To analyze the differences of the serum levels of Th1-type cytokine IFN-γ and Th2-type cytokine IL-4 in patients with ovarian cancer and benign ovarian tumors, as well as the differential expression of mucin-1(MUC1) and breast cancer 1-associated protein-1(BAP1) in lesion tissue between the two kinds of patients. A total of 60 ovarian cancer patients admitted to Qingdao Women and Children’s Hospital from 2015 to 2022 were selected,50 patients with benign ovarian tumors who were surgically treated and diagnosed by pathology during the same period were selected, and 40 healthy women who underwent physical examination during the same period were selected. The levels of IFN-γ and IL-4 were detected by enzyme-linked immunosorbent assay, and the expressions of MUC1 and BAP1 in the lesion tissue were detected by immunohistochemistry;the diagnostic value of IFN-γ and IL-4 in patients with ovarian cancer was analyzed by ROC curve. Postoperative pathological tissues were collected for detecting the positive expression of MUC1 and BAP1,while their relationship with pathological characteristicsand tumor malignancy were analyzed. Data showed that the level of serum IFN-γ in ovarian cancer group was lower than those in benign ovarian tumor group and healthygroup, while the level of IL-4 was higher than those in benign ovarian tumor group and healthy group(P<0.05). Thelevels of serum IFN-γ and IL-4 did not showe statistically difference between benign ovarian tumor group andhealthy group(P>0.05). The ROC curve showed that the AUC of IFN-γ and IL-4 were 0.748 and 0.796, thesensitivity was 98.2% and 80.9%, and the specificity was 40.0% and 62.5%, respectively. The positive expressionrates of MUC1 and BAP1 in ovarian cancer tissues were higher than those in benign ovarian tumors(P<0.05);thepositive expression of MUC1 and BAP1 showed no statistical difference in patients of different age and tumordiameter(P>0.05), but demonstrated significant differences in patients with different FIGO stages, pathologicalgrades, and lymph node metastasis(P<0.05);Spearman analysis showed that the expression of MUC1 and BAP1were positively correlated with FIGO stage, pathological grade and lymph node metastasis(P<0.05). Taken together,the levels of IFN-γ and IL-4 in serum and the positive degree of MUC1 and BAP1 in lesion tissue may be used asauxiliary diagnostic indicators for ovarian cancer.
作者
阿艳妮
王晓燕
A Yanni;WANG Xiaoyan(Department of Gynecology,Qingdao Women and Children's Hospital,Qingdao 266000,China;Department of Reproduction,Qingdao Women and Children's Hospital,Qingdao 266000,China)
出处
《免疫学杂志》
CAS
CSCD
北大核心
2022年第12期1074-1079,共6页
Immunological Journal