污水处理厂生物气溶胶抗生素抗性污染特征

Pollution characteristics of antibiotics resistance associated with bioaerosols from a wastewater treatment plant

为探究污水处理厂生物气溶胶抗生素抗性基因(ARGs)污染特征,在济南市某污水处理厂采用宏基因组测序技术对厂界内及周边生物气溶胶样本及污水或污泥样本进行分析.结果表明,相比于上风向,厂界内和下风向生物气溶胶具有更多的ARGs亚型种类数和更高的总相对丰度.厂界内与上风向生物气溶胶ARGs组成存在显著的差异性,差异度为47.57%;而厂界内与下风向生物气溶胶ARGs组成的差异性不显著,且差异度下降至33.98%.上风向背景空气和污水或污泥均是厂界内生物气溶胶ARGs的重要来源,两者总的源的贡献大于63.92%.共检测到43种ARGs亚型(8种ARGs主型)在至少一处污水处理单元极易负载于生物气溶胶颗粒逸出.本研究可为污水处理厂生物气溶胶抗生素抗性污染的风险评估和控制提供理论依据.

In order to explore the pollution characteristics of antibiotic resistance genes(ARGs)associated with bioaerosols from a wastewater treatment plant in Jinan,bioaerosol within and around this plant,and wastewater or sludge,were analyzed using shotgun metagenome sequencing.Compared to bioaerosols from upwind,that from this plant and downwind were characterized with more subtype number and higher total abundance of ARGs.Significant difference(47.57%)for the compositions of ARGs in bioaerosols,was found between upwind and this plant.The dissimilarity of ARGs in bioaerosols was not significant between downwind and this plant,and the difference decreased to 33.98%.Ambient air and wastewater/sludge were both the important source for ARGs in bioaerosols from this plant,with the total source contribution higher than 63.92%.A total of 43subtypes(8types)of ARGs were easily aerosolized in bioaerosols from at least one of the treatment units.These results could provide the theoretical basis for the risk assessment and control of antibiotic resistant contamination in wastewater treatment plants.