双光子钙成像技术在啮齿类动物前边缘皮层中的应用研究进展

Research Progress in Application of Two-Photon Calcium Imaging in Prelimbic Cortex of Rodents

钙离子是生物体内重要信使之一,细胞钙信号与神经活动和功能有关,钙信号的监测技术有助于对神经机制的研究。双光子钙成像技术的出现是钙成像领域的重大进步,该技术可以对活体动物成像,能够在细胞和亚细胞层次对神经元进行研究,其大范围的细胞分辨率成像能够同时记录数百个细胞,为神经科学研究提供便利。啮齿类动物的前边缘皮层与人类背外侧前额叶的功能相似,对啮齿类动物前边缘皮层进行双光子钙成像有助于加深对人类背外侧前额叶神经机制的了解。文章对双光子钙成像在啮齿动物前边缘皮层中的应用研究进展进行综述。

Calcium ion is one of the important messengers in organisms.Cellular calcium signal is related to neural activity and function,and the monitoring of calcium signal is helpful for the study of neural mechanisms.The emergence of two-photon calcium imaging is a major advance in the field of calcium imaging.The technology allows imaging of living animals and can study neurons at the cellular and subcellular levels.Its wide range of cell-resolution imaging can record hundreds of cells simultaneously,facilitating neuroscience research.The prelimbic cortex in rodents is functionally similar to the dorsolateral prefrontal cortex in humans.Therefore,the two-photon imaging of rodent prefrontal cortex is helpful to understand the neural mechanisms of human dorsolateral prefrontal cortex.This paper reviews the application of two-photon calcium imaging in rodent prelimbic cortex.