宏基因组二代测序在儿童急性白血病化疗后合并毛霉菌病早期诊断中的应用价值

Application of Metagenomics Next Generation Sequencing(mNGS)in detection of mucormycosis after chemotherapy in childhood acute leukemia

目的通过分析应用宏基因组二代测序(mNGS)方法检测儿童急性白血病化疗后合并毛霉菌病的病例情况,探讨这种检测方法对于儿童急性白血病化疗后合并毛霉菌病的早期诊断的应用价值。方法回顾性分析2020年1月—2021年12月收治于河北省儿童医院血液肿瘤科的7例急性白血病化疗后合并毛霉菌病患儿的临床资料,总结其临床特点,诊疗经过及转归。结果7例患儿中6例为急性淋巴细胞白血病,1例为急性髓系白血病,均经过mNGS检测出毛霉菌感染,其中1例为肺脑型,6例为肺型,经治疗,1例死亡,2例治愈,4例好转。结论mNGS检测方法在儿童急性白血病化疗后合并毛霉菌病的早期诊断中具有重要参考价值。

Objective To analyze the application of Metagenomics next-generation sequencing(mNGS)in detection of mucormycosis after chemotherapy in childhood acute leukemia,and to explore the value of early detection of this method in this case.Methods To retrospectively analyze the clinical data of 7 children with acute leukemia combined with mucormycosis after chemotherapy in the Department of Hematology and Oncology,Hebei Children′s Hospital from January 2020 to December 2021,and summarize their clinical characteristics,diagnosis and treatment process and outcome.Results Among the 7 children,6 cases were acute lymphoblastic leukemia and 1 case was acute myeloid leukemia.Mucor infection was detected by mNGS,among which 1 case was pulmonary brain type and 6 cases were pulmonary type.After treatment,1 case died,2 cases were cured and 4 cases were improved.Conclusions The mNGS detection has important value in early diagnosis of mucormycosis after chemotherapy in children with acute leukemia.