肺癌组织中USP3及FOXF1表达与临床特征的关系及其与肺癌组织EMT标识物的相关性

The Relationship between the Expressions of USP3 and FOXF1 and Clinical Features in Lung Cancer Tissues and Their Correlation with Epithelial-mesenchymal Transition Markers in Lung Cancer Tissue

目的:探究肺癌组织中去泛素化酶3(USP3)、叉头蛋白1(FOXF1)表达与临床特征的关系及其与肺癌组织上皮间质转化(EMT)标识物的相关性。方法:回顾性分析2016年1月-2019年12月南通大学附属医院收治的130例肺癌患者的临床资料,并取患者手术切除的癌组织及其周围的癌旁组织(均经病理诊断)。采用免疫组化法检测患者癌组织及癌旁组织中USP3、FOXF1的阳性表达情况;分析肺癌组织中USP3及FOXF1表达与临床特征的关系;并通过Spearman相关分析探究USP3、FOXF1的表达与肺癌组织EMT标识物上皮型钙黏附素(E-cardherin)和波形蛋白(Vimentin)的相关性。结果:130例肺癌组织标本中USP3及FOXF1阳性表达率分别为92.31%(120/130)、86.15%(112/130),癌旁组织中USP3及FOXF1阳性表达率分别为31.54%(41/130)、29.23%(38/130),肺癌组织的USP3及FOXF1阳性表达率均高于癌旁组织(P<0.05)。不同USP3及FOXF1表达情况患者的性别、年龄及病理类型比较,差异均无统计学意义(P>0.05)。不同USP3及FOXF1表达情况患者的临床分期比较,差异均有统计学意义(P<0.05)。130例肺癌组织样本中Vimentin阳性81例,阳性表达率为62.31%,E-cadherin阴性90例,阴性表达率为69.23%;经Spearman分析,USP3、FOXF1与Vimentin均呈正相关,USP3、FOXF1与E-cadherin均呈负相关(P<0.05)。结论:USP3、FOXF1在肺癌组织中表达明显,与肺癌患者临床特征具有一定的联系。同时USP3、FOXF1与肺癌细胞EMT标识物具有明显的相关性,与肺癌的发展密切相关。

Objective:To explore the relationship between the expressions of Ubiquitin-specific protease 3(USP3)and forkhead protein 1(FOXF1)and clinical features in lung cancer tissues and their correlation with epithelial-mesenchymal transition(EMT)markers in lung cancer tissues.Method:The clinical data of 130patients with lung cancer admitted to Affiliated Hospital of Nantong University from January 2016 to December 2019 were retrospectively analyzed,and the surgically removed cancer tissues and their surrounding paracancer tissues(all pathologically diagnosed)were taken.The positive expressions of USP3 and FOXF1 in the cancer tissues and paracancer tissues of patients were detected by immunohistochemical method;the relationship between the expressions of USP3 and FOXF1 and clinical features in lung cancer tissues were analyzed;and the correlation between the expressions of USP3 and FOXF1 and EMT markers E-cardherin and Vimentin in lung cancer tissues were explored by Spearman correlation analysis.Result:The positive expression rates of USP3 and FOXF1 in 130 lung cancer tissues were 92.31%(120/130)and 86.15%(112/130),and 31.54%(41/130),29.23%(38/130)in paracancer tissues,respectively,the positive expression rates of USP3 and FOXF1 in lung cancer tissues were higher than those in paracancer tissues(P<0.05).There were no significant differences in gender,age and pathological type of patients with different expressions of USP3 and FOXF1(P>0.05).There was significant difference in clinical stages of patients with different expressions of USP3 and FOXF1(P<0.05).Among 130 lung cancer tissue samples,81 cases were Vimentin positive(62.31%),and 90 cases were E-cadherin negative(69.23%).Spearman analysis showed that USP3 and FOXF1 were positively correlated with Vimentin,while USP3 and FOXF1 were negatively correlated with E-cadherin(P<0.05).Conclusion:USP3 and FOXF1 are significantly expressed in lung cancer tissues,and have a certain relationship with the clinical characteristics of lung cancer patients.At the same time,USP3 and FOXF1 have obvious correlations with EMT markers of lung cancer cells,and are closely related to the development of lung cancer.