急性胰腺炎患者血清miR-181a-5p表达及与Th17/Treg免疫平衡的关系

Relationship of Serum miR-181a-5p Expression with Th17/Treg Immune Homeostasis in Patients with Acute Pancreatitis

目的:探讨急性胰腺炎(AP)患者血清微小RNA-181a-5p(miR-181a-5p)的表达及与辅助性T细胞17/调节性T细胞(Th17/Treg)免疫平衡的关系。方法:选取2020年1月至2021年12月我院收治的110例AP患者(AP组),根据病情严重程度分为轻症AP组41例、中度重症AP组37例、重症AP组32例,另选取同期58名体检健康者(对照组)。采用酶联免疫吸附法检测外周血白细胞介素-6(IL-6)、IL-10、IL-17、转化生长因子-β1(TGF-β1)水平,流式细胞术检测外周血Th17、Treg百分比,qPCR检测血清miR-181a-5p表达。通过Pearson/Spearman相关性分析AP患者血清miR-181a-5p表达与Th17/Treg的相关性。结果:与对照组比较,AP组血清miR-181a-5p表达和外周血Treg百分比降低,Th17百分比和Th17/Treg升高(P均<0.001)。与对照组比较,AP组外周血IL-6、IL-17水平升高,IL-10、TGF-β1水平降低(P均<0.001)。轻症、中度重症、重症AP组血清miR-181a-5p表达和外周血IL-10、TGF-β1、Treg百分比依次降低,外周血IL-6、IL-17、Th17百分比、Th17/Treg依次升高(P均<0.001)。Pearson/Spearman相关性分析显示,AP患者血清miR-181a-5p表达与IL-6、IL-17、Th17、Th17/Treg呈负相关(r/rs=-0.688、-0.652、-0.782、-0.825,P均<0.001),与IL-10、TGF-β1、Treg呈正相关(r/rs=0.743、0.667、0.739,P均<0.001)。结论:AP患者血清miR-181a-5p低表达,Th17/Treg免疫失衡,miR-181a-5p可能通过Th17/Treg免疫失衡参与AP发生发展。

Objective:To investigate the expression of serum microRNA-181a-5p(miR-181a-5p)in patients with acute pancreatitis(AP)and the relationship with T helper cell 17/T regulatory cell(Th17/Treg)immune homeostasis.Methods:110 patients with AP admitted to our hospital from January 2020 to December 2021(AP group)were selected and divided into the mild AP group(n=41),the moderately severe AP group(n=37)and the severe AP group(n=32)according to the severity of their conditions,and 58 healthy people with physical examination(control group)were also selected for the same period.Peripheral blood interleukin-6(IL-6),IL-10,IL-17 and transforming growth factor-β1(TGF-β1)levels were measured by enzyme-linked immunosorbent assay,peripheral blood Th17 and Treg percentages were measured by flow cytometry,and serum miR-181a-5p expression was measured by qPCR.Correlation of serum miR-181a-5p expression with Th17/Treg in AP patients was analyzed using Pearson/Spearman correlation.Results:Compared with the control group,serum miR-181a-5p expression and peripheral blood Treg percentage decreased,and Th17 percentage and Th17/Treg increased in AP group(all P<0.001).Compared with the control group,the peripheral blood levels of IL-6 and IL-17 in the AP group were increased,and the levels of IL-10 and TGF-β1 were reduced(all P<0.001).Serum miR-181a-5p expression and peripheral blood IL-10,TGF-β1,and Treg percentage were sequentially lower and peripheral blood IL-6,IL-17,Th17 percentage,and Th17/Treg were sequentially higher in the mild,moderately severe,and severe AP groups(all P<0.001).Pearson/Spearman correlation analysis showed that serum miR-181a-5p expression in AP patients was negatively correlated with IL-6,IL-17,Th17,and Th17/Treg(r/rs=-0.688,-0.652,-0.782,-0.825,all P<0.001)and positively correlated with IL-10,TGF-β1,and Treg were positively correlated(r/rs=0.743,0.667,0.739,all P<0.001).Conclusion:Serum miR-181a-5p is lowly expressed in AP patients with Th17/Treg immune imbalance.miR-181a-5p may be involved in the development of AP through Th17/Treg immune imbalance.