摘要
采用β环糊精敏化荧光法测定黄柏提取液中盐酸小檗碱的含量.盐酸小檗碱是黄柏的主要提取物,与β环糊精可以形成包合物,产生敏化荧光作用.在7.50mg/mLβ环糊精水溶液中,选择荧光激发波长(λex)345nm,发射波长(λem)540nm,工作曲线线性范围0.10~1.00μg/mL,检出限1.33ng/mL,测得黄柏中盐酸小檗碱的含量为1.56%.此方法操作简单、准确,对中药中盐酸小檗碱的含量测定具有一定的参考价值.
The content of berberine hydrochloride in phellodendron extracting solution was determined byβ-cyclodextrin sensitized fluorescence method.Berberine hydrochloride is the main extract of phellodendron,which can form inclusion complex withβ-cyclodextrin to generate fluorescence sensitization.In 7.50 mg/mLβ-cyclodextrin aqueous solution,the excitation wavelength(λex)and emission wavelength(λem)of the fluorescence detection were 345 and 540 nm,respectively.The linear range of the working curve was 0.10 to 1.00μg/mL,and the detection limit was 1.33 ng/mL.The results showed that the content of berberine hydrochloride in phellodendron was 1.56%.This method is simple and accurate,and has certain reference value for the determination of berberine hydrochloride in traditional Chinese medicine.
作者
郑彦慧
陈鹏
曹津津
ZHENG Yanhui;CHEN Peng;CAO Jinjin(Shijiazhuang Vocational College of Finance and Economics,Hebei Shijiazhuang 050061,China;College of Chemistry and Materials Science,Hebei Normal University,Hebei Shijiazhuang 050024,China;Shijiazhuang Productivity Promotion Service Center,Hebei Shijiazhuang 050090,China;Department of Environmental and Chemical Engineering,Hebei Vocational University of Industry and Technology,Hebei Shijiazhuang 050091,China)
出处
《河北师范大学学报（自然科学版）》
CAS
2023年第1期74-78,共5页
Journal of Hebei Normal University：Natural Science
基金
河北省“三三三人才工程”资助项目(A202101097)
河北省社会科学发展研究课题(20220202378)
河北省高等学校自然科学研究项目(Z2020138)
河北工业职业技术学院博士基金(bz2020004)。
关键词
黄柏
盐酸小檗碱
敏化荧光法
Β环糊精
检出限
phellodendron
berberine hydrochloride
sensitized fluorescence method
β-cyclodextr in
detection limit
