液液微萃取结合GC-MS-SIM法检测酱香型白酒中15种微量成分含量

Determination of Fifteen Trace Components in Jiangxiang Baijiu by Liquid-Liquid Microextraction Combined with GC-MS-SIM

利用液液微萃取结合气相色谱-质谱-选择离子扫描(gas chromatography-mass spectrometryselective ion monitoring,GC-MS-SIM)检测12个酱香型白酒中9种吡嗪和6种酚类化合物的含量。结果表明,15种微量成分在各自范围内均具有良好的线性关系(R^(2)>0.9990),加标回收率在84.23%~98.85%之间,相对标准偏差均低于5.00%。检测结果表明,四甲基吡嗪是含量最高的吡嗪类物质,2,6-二甲基吡嗪、三甲基吡嗪等含量相对较高,所有酒样中吡嗪类物质含量范围为1.00~11.89 mg/L,不同酒样中含量差异较为显著;酚类物质中含量最高的是苯酚,所有酒样含量范围为0.32~0.84 mg/L。该方法样品用量少、处理简单、速度快且检测结果可靠,为酱香型白酒中吡嗪和酚类化合物的快速检测提供了参考和借鉴。

A total of nine pyrazines and six phenolic compounds in 12 Jiangxiang Baijiu samples were analyzed by liquid-liquid microextraction combined with gas chromatography-mass spectrometry-selective ion monitoring(GC-MS-SIM).The results showed that the 15 trace components displayed a good linearity(R^(2)>0.9990)in their respective ranges,the recoveries were in the range of 84.23%to 98.85%,and the relative standard deviations were less than 5.00%.Tetramethylpyrazine had the highest content among the pyrazines,followed by 2,6-dimethylpyrazine and trimethylpyrazine.The concentration range of pyrazines in all samples was between1.00 mg/L and 11.89 mg/L,and the concentration of pyrazines was significantly different in different samples.Phenol had the highest content among the phenolic compounds.The concentration range of phenolic compounds in all samples was between 0.32 mg/L and 0.84 mg/L.The method has the advantages of low sample consumption,simple operation,fast speed and reliable results,which provides reference for the rapid detection of pyrazines and phenolic compounds in Jiangxiang Baijiu.