miR-103a-2、miR-122和vaspin在非酒精性脂肪性肝病合并HBV感染中的表达及意义

Significance of expression of miR-103a-2,miR-122,and vaspin in non-alcoholic fatty liver disease with HBV infection

目的探讨微小RNA(miR)-103a-2、miR-122和丝氨酸蛋白酶抑制剂(vaspin)在非酒精性脂肪肝病(NAFLD)合并乙型肝炎病毒(HBV)感染中的表达及意义。方法选择东营市胜利油田中心医院于2019年5月至2021年5月收治的NAFLD合并HBV感染患者97例作为观察组;选择同期收治的单纯NAFLD患者82例作为NAFLD组;另选择健康体检者80例作为正常组。所有研究对象均以实时荧光定量法(qRT-PCR)法检测miR-103a-2和miR-122相对表达量;采用酶联免疫吸附法(ELISA)测定血清vaspin水平;采用全自动生化分析仪测定肝功能[总胆红素(TBIL)、天门冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)]水平。比较各组miR-103a-2和miR-122相对表达量,TBIL、AST和ALT水平变化。采用Spearson分析miR-103a-2、miR-122和vaspin与肝功能相关性;采用多因素Logistic回归分析NAFLD合并HBV感染影响因素。结果观察组miR-103a-2和miR-122相对表达量及vaspin水平高于NAFLD组和正常组(P<0.05);NAFLD组miR-103a-2和miR-122相对表达量及vaspin水平高于正常组(P<0.05)。观察组血清TBIL、AST和ALT水平高于NAFLD组和正常组(P<0.05);NAFLD组血清TBIL、AST和ALT水平高于正常组(P<0.05)。采用Spearson相关性分析显示,miR-103a-2、miR-122和vaspin与呈TBIL、AST和ALT呈正相关(P<0.05)。采用多因素Logistic回归分析显示,miR-103a-2、miR-122和vaspin为影响NAFLD合并HBV感染独立危险因素。结论miR-103a-2、miR-122和vaspin在NAFLD合并HBV感染患者中高表达,且为影响NAFLD合并HBV感染独立危险因素。

Objective To investigate the expression of microRNA(miR)-103a-2,miR-122,and(vaspin)in non-alcoholic fatty liver disease(NAFLD)combined with hepatitis B virus(HBV)infection and analyze their significance.Methods A total of 97 NAFLD patients with HBV infection admitted to Dongying Shengli Oilfield Central Hospital from May 2019 to May 2021 were selected as an observation group,82 patients with simple NAFLD were selected as an NAFLD group,and another 80 healthy subjects were selected as a normal group.The relative expression levels of miR-103A-2 and miR-122 were detected by real-time fluorescence quantitative assay(qRT-PCR).Serum vaspin level was determined by enzyme-linked immunosorbent assay(ELISA).An automatic biochemical analyzer was used to determine liver function indexes[total bilirubin(TBIL),aspartate aminotransferase(AST),and alanine aminotransferase(ALT)].The relative expression levels of miR-103A-2 and miR-122 and the changes of TBIL,AST,and ALT levels in each group were compared.Spearman analysis was used to analyze the correlation of miR-103a-2,miR-122,and vaspin expression with liver function.Multivariate logistic regression was used to analyze the influencing factors of NAFLD combined with HBV infection.Results The relative expression levels of miR-103a-2 and miR-122 as well as serum vaspin level were significantly higher in the observation group than in the NAFLD group and normal group(P<0.05),and in the NAFLD group than in the normal group(P<0.05).Serum TBIL,AST,and ALT levels were significantly higher in the observation group than in the NAFLD group and normal group(P<0.05),and in the NAFLD group than in the normal group(P<0.05).Spearman correlation analysis showed that miR-103a-2,miR-122,and vaspin were positively correlated with serum levels of TBIL,AST,and ALT(P<0.05).Multivariate logistic regression analysis showed that miR-103a-2,miR-122,and vaspin were independent risk factors affecting NAFLD combined with HBV infection.Conclusion miR-103a-2,miR-122,and vaspin are highly expressed in NAFLD patients with HBV infection,and are independent risk factors for NAFLD with HBV infection.