兼具抗菌及促细胞黏附特性的钛表面光热涂层的研究

Study of photothermal coating on titanium surface with antibacterial and cell adhesive properties

目的构建兼具抗菌及促进细胞黏附的钛表面光热涂层,并探讨其临床应用潜力。方法通过将光热聚多巴胺纳米颗粒涂层(PDA-NP)应用于需要组织整合的钛基植入物表面以控制感染。通过细菌和细胞共培养的模型评估钛表面的PDA-NP涂层杀菌效果和细胞黏附效果。结果体外生物学结果显示,PDA-NP涂层明显增强了细胞的黏附,有利于细胞增殖。对具有PDA-NP涂层的钛表面进行NIR辐照,约3 min即可杀死黏附的金黄色葡萄球菌(S.aureus)。另外,具有PDA-NP涂层的钛表面被S.aureus污染后,接种成骨细胞MC3T3-E1,辐照约3 min后,细菌仍可被有效杀死,且MC3T3-E1的细胞黏附行为不受影响。结论钛植入物表面构建PDA-NP光热涂层能够显著抗菌,控制感染,并促进细胞黏附,从而促进组织整合,防止感染部位周围组织的附带损伤。

Objective To construct a photothermal coating on a titanium surface with antibacterial cell adhesive properties,and to discuss its potential for clinical application.Methods Photothermal polydopamine nanoparticle(PDA-NP)coatings were smeared on the surface of titanium-based biomaterials that required tissue integration to control infection.The antibacterial effect and cell adhesion of PDA-NP coating on titanium surfaces were evaluated by bacterial and cell co-culture models.Results In vitro results showed that PDA-NP coating significantly enhanced cell adhesion and was conducive to cell proliferation.The adhered Staphylococcus aureus(S.aureus)on the titanium surface with PDA-NP coating could be killed by near-infrared(NIR)irradiation in 3 minutes.Besides,when MC3T3-E1 osteoblasts were inoculated on the titanium surface with PDA-NP coating after contaminated with S.aureus,the bacteria could be effectively killed after about 3minutes of NIR irradiation with nearly no impairment to the cell adhesion behavior of MC3T3-E1.Conclusion The PDANP photothermal coatings on the surface of titanium implants are significantly antimicrobial to control infection and promote cell adhesion,thereby promoting tissue integration and preventing collateral damage to the surrounding tissue at the site of infection.