胜红蓟内生真菌转化柠檬烯产物的抑菌活性

Antibacterial Activity of Limonene Product Transformed by Ageratum conyzoides Endophytic Fungi

为考察入侵植物胜红蓟内生真菌转化柠檬烯产物的抑菌活性,以柠檬烯二醇作为标品对照,采用薄层色谱法筛选具有转化柠檬烯能力的胜红蓟内生真菌.通过气相色谱-质谱法对菌株的转化产物进行分析,并对其生物转化柠檬烯发酵液乙酸乙酯粗提物的抑菌活性进行研究.从22株胜红蓟内生真菌中共筛选得到14株具有转化柠檬烯能力的菌株,其中,有6个菌株的柠檬烯转化乙酸乙酯粗提物中含有柠檬烯-1,2-二醇;菌株Diaporthe phaseolorum(wl01)的柠檬烯转化产物中,柠檬烯-1,2-二醇的质量浓度最高(3.43 mg·mL^(-1));菌株Alternaria alternata(wp06)生物转化柠檬烯发酵液的乙酸乙酯提取物对柑橘意大利青霉类致病菌有一定的抑制效果,对意大利青霉的抑菌效果为(40.03±3.58)%.

In order to explore the antibacterial activity of limonene conversion by endophytic fungi from invasive plant Ageratum conyzoides L.,using limonene-1,2-diol as the standard control,the endophytic fungi with the ability to transform limonene were screened by thin layer chromatography.The transformation products of the strain were analyzed by gas chromatography-mass spectrometry,and the antibacterial activity of the ethyl acetate extracts of the biotransformed limonene fermentation broth was studied.It was found that 14 strains with the ability to transform limonene were screened from 22 Ageratum conyzoides L.endophytic fungi,among them,6 strains of limonene-converted ethyl acetate crude extracts contained limonene-1,2-diol.The mass concentration of limonene-1,2-diol in the limonene conversion product of strain Diaporthe phaseolorum(wl01)was the highest(3.43 mg·mL^(-1)).The ethyl acetate extract of strain Alternaria alternata(wp06)biotransformed limonene fermentation broth had a certain inhibitory effect on Penicillium italicum,and the inhibitory effect on Penicillium italicum was(40.03±3.58)%.