芪蛭通窍胶囊对外源性睾酮介导大鼠前列腺增生的抑制作用及机制探讨

Exploration of the inhibitory effects and mechanism of Qizhi Tongqiao capsule on exogenous testosterone-induced benign prostatic hyperplasia in rats

目的探索芪蛭通窍胶囊对外源性睾酮所致大鼠前列腺增生的抑制作用及机理。方法27只雄性SD大鼠随机均分为3组:正常对照组、良性前列腺增生模型组和芪蛭通窍胶囊治疗组。经皮下注射丙酸睾酮(5 mg/kg/天)构建良性前列腺增生大鼠模型,芪蛭通窍胶囊粉末混悬液(1.52 g/kg/天)灌胃28天后处死大鼠,记录体重、前列腺湿重,取前列腺组织。HE染色法检测前列腺组织病理改变。Western blotting检测前列腺组织中Bax、Bcl-2、PCNA、HIF-1α、VEGF蛋白表达。结果与对照组比较,模型组大鼠前列腺湿重和前列腺指数均显著升高(P<0.01),芪蛭通窍胶囊干预后可抑制该现象(分别为P<0.01,P<0.05)。HE染色结果显示,与对照组相比,模型组大鼠前列腺上皮显著增厚(P<0.01),且腺腔面积变小,芪蛭通窍胶囊干预后可明显抑制前列腺上皮增厚(P<0.01)。与对照组比较,模型组大鼠前列腺组织中Bcl-2、PCNA、HIF-1α及VEGF蛋白含量均显著升高(P<0.01),Bax蛋白含量和Bax/Bcl-2比例均显著下降(P<0.01),芪蛭通窍胶囊干预后可显著抑制Bcl-2、PCNA、HIF-1α和VEGF蛋白表达(P<0.01),并上调Bax/Bcl-2比例(P<0.01)。结论芪蛭通窍胶囊可显著抑制外源性睾酮介导的大鼠前列腺增生,改善前列腺组织病理变化,抑制前列腺上皮增厚,其起效机理可能与抑制前列腺组织中Bcl-2、PCNA、HIF-1α、VEGF蛋白表达水平及提高Bax/Bcl-2比值有关。

Objective To explore the inhibitory effects of Qizhi Tongqiao capsule(QTC)on rat prostatic hyperplasia induced by exogenous testosterone propionate and mechanisms.Methods A total of 27 male SD rats were included in this study and divided into 3 groups randomly and averagely as normal control group,benign prostatic hyperplasia(BPH)model group and QTC treatment group.BPH rat models were established through subcutaneous injection with testosterone propionate(5mg/kg/d).Powder suspension of QTC was given orally for 28 days(1.52g/kg/d),then rats were all executed.Body weight and prostatic wet weight were recorded and prostate tissues were obtained.Pathological changes of the rat prostate were examined by HE staining.Protein expressions of Bax,Bcl-2,PCNA,HIF-1αand VEGF were tested through Western blotting.Results Compared with the control group,the prostatic wet weight and prostatic index of rats in the model group both elevated significantly(P<0.01)which can be inhibited by orally-administered QTC(P<0.01,P<0.05).HE staining results indicated in the model group the prostatic epithelial became thicker(P<0.01)and lumen area decreased than in the control group,which can be improved by orally-administered QTC(P<0.01).Compared with the control group,protein expressions of Bcl-2,PCNA,HIF-1αand VEGF significantly increased while protein expression of Bax and Bax/Bcl-2 ratio significantly decreased in the prostatic tissues of rats in the model group(P<0.01).However,protein expressions of Bcl-2,PCNA,HIF-1αand VEGF can be significantly inhibited and Bax/Bcl-2 ratio was markedly elevated with QTC treatment(P<0.01).Conclusion QTC treatment can significantly impede rat BPH which was induced by subcutaneous injection with testosterone propionate and ameliorate pathologic changes of the prostate,inhibit prostatic epithelial thickening.And the inhibitory effects on Bcl-2,PCNA,HIF-1α,VEGF protein expressions and improving effect on Bax/Bcl-2 ratio of QTC may partly explain why it works.