菟丝子-枸杞子药对调控PI3K/Akt通路改善精原细胞功能障碍的实验研究

Effects of Semen Cuscutae-Lycium barbarum on improving spermatogonia dysfunction via regulating PI3K/Akt pathway

目的探讨菟丝子枸杞子药对对生精功能障碍精原细胞增殖、凋亡的改善作用以及对PI3K/Akt通路蛋白表达的影响,为补肾生精法治疗男性不育症提供理论支撑。方法正常SD大鼠20只,随机分为空白对照组、雷公藤多苷组、左卡尼汀组、菟丝子枸杞子组,用以制备含药血清,并分别干预精原细胞,观察PI3K/Akt通路相关蛋白改变。结果与雷公藤多苷组相比,菟丝子枸杞子药对能提高PI3K、p-AKT、Bcl-2蛋白及mRNA表达(P<0.05或0.01),下调BAD、BAX蛋白及mRNA表达(P>0.05或0.01)。结论菟丝子枸杞子药对能改善精原细胞功能障碍,改善作用可能与调节PI3K/Akt通路有关。

Objective To investigate the effects of Semen Cuscutae-Lycium barbarum pair on spermatogonia proliferation and apoptosis,as well as PI3K/Akt pathway activation for providing a theoretical support of supplementing kidney spermatogenic treatment on male infertility.Methods A total of 20 normal SD rats were randomly divided into four groups such as the blank control group,the Tripterygium wilfordii multi-glycoside group,the levocarnitine group,and Semen Cuscutae-Lycium barbarum group.Serum containing medicine were respectively collected from the treated rats in four groups,and was used to intervene spermatogonia for observing PI3K/Akt pathway activation.Results Compared with those in the Tripterygium wilfordii multi-glycoside group,the expressions of PI3K,p-Akt and bcl-2 were significant upregulated in the Semen Cuscutae-Lycium barbarum group(P<0.05 or 0.01),whereas the expressions of BAD,BAX were downregulated(P>0.05 or 0.01).Conclusion Semen Cuscuta-lycium barbarum pair can improve spermatogonia dysfunction,and its underlying mechanism may be related to PI3K/Akt pathway activation.