4-氨基吡啶对MPP+诱导帕金森病细胞具有神经保护作用

Neuroprotective effect of 4-aminopyridine in 1-methyl 4-phenylpyridinium induced Parkinson’s disease cells model

目的探究4-氨基吡啶(4-aminopyridine,4-AP)对1-甲基-4-苯基吡啶离子(1-methyl-4-phenylpyridinium,MPP+)诱导的帕金森病细胞模型是否存在保护作用。方法利用不同浓度的MPP+孵育SH-SY5Y细胞,探究不同浓度MPP+对细胞活性的影响。单独利用4-AP孵育细胞,探究4-AP是否影响细胞活性。利用4-AP预处理SH-SY5Y细胞系24h后,随后选取合适浓度的MPP+孵育细胞24h,CCK8测定细胞活性并进行统计学分析。结果随着MPP+药物浓度的升高,SH-SY5Y细胞活性下降。不同浓度的4-AP单独孵育SH-SY5Y细胞系对细胞活性不产生影响。不同浓度的4-AP预处理细胞24h后,加入1mol/L的MPP+孵育24h,当4-AP浓度在0.3~10mol/L时SH-SY5Y细胞的活性增加,在1mol/L时细胞活性增加最佳,为正常细胞的66%。结论一定浓度范围内的4-AP可减少MPP+对SH-SY5Y细胞的毒性作用,具有神经保护作用。

Objective To explore the protective effect of 4-aminopyridine(4-AP)on 1-methyl-4-phenylpyridinium(MPP+)induced Parkinson’s disease cell mode.Methods SH-SY5Y cells were incubated with different concentrations of MPP+to explore the effect of different concentrations of MPP+on cell viability.SH-SY5Y cells were incubated with different concentrations of 4-AP to explore the effect of different concentrations of drugs on cells.SH-SY5Y cells were pretreated with 4-AP for 24h,then incubated with MPP+at an appropriate concentration for 24h,and cell viability was measured by CCK8 assay.Results With the increase of MPP+concentration,the activity of SH-SY5Y cells decreased.Different concentrations of 4-AP alone incubating SH-SY5Y cell line had no effect on cell viability.After the cells were pretreated with different concentrations of 4-AP for 24 h,and then incubated with 1 mol/L MPP+for 24 h,the activity of SH-SY5Y cells increased when the concentration of 4-AP was 0.3-10mol/L,and the best increase was 66%of the control cells when the concentration of 4-AP was 1mol/L.Conclusion In a certain concentration range,4-AP can reduce the toxic effect of MPP+on SH-SY5Y cells and has neuroprotective effect.