糖肝煎对2型糖尿病大鼠摄食及摄食抑制因子1、饥饿素的影响

Effect of Tanggan decoction on food intake,Nesfatin-1 and Ghrelin in type 2 diabetic rats

目的探究糖肝煎对2型糖尿病(T2DM)大鼠摄食及血浆摄食抑制因子1(Nesfatin-1)、饥饿素(Ghrelin)的影响。方法将50只SD大鼠采用随机数字表法分成正常组、T2DM组、利格列汀组(50 mg/kg利格列汀)、低剂量组(2.8 g/kg糖肝煎)、高剂量组(5.6 g/kg糖肝煎),10只/组;通过尾静脉注射链脲佐菌素(STZ)及高脂饲料进行T2DM大鼠模型构建;利格列汀组、低剂量组及高剂量组连续4周给予相应药物灌胃,正常组及T2DM组给予等量生理盐水,1次/天,于实验结束后观察大鼠一般情况,并称取大鼠体质量,测定摄食量、摄水量、尿量;全自动生化分析仪测定大鼠血清空腹血糖(FBG)、低密度脂蛋白胆固醇(LDLC)、高密度脂蛋白胆固醇(HDL-C)、三酰甘油(TG)及总胆固醇(TC)水平;计算大鼠口服葡萄糖耐量试验(OGTT)曲线下面积(AUC);酶联免疫吸附试验(ELISA)检测血清胰岛素(INS)水平及血浆核连蛋白2(NUCB2)、Ghrelin、Nesfatin-1水平;实时荧光定量逆转录聚合酶链式反应(qRT-PCR)检测血浆Nesfatin-1、Ghrelin mRNA水平。结果正常组大鼠粪便正常,皮毛光泽顺滑,行动灵活、精神状态良好;T2DM组大鼠出现便溏,精神不振、皮毛发黄粗糙;相较于T2DM组,利格列汀组、低剂量组、高剂量组大鼠上述症状存在不同程度改善;相较于正常组,T2DM组大鼠血清FBG水平、OGTT AUC、摄食量、摄水量、尿量、LDL-C、TG、TC、Ghrelin水平显著增加,INS水平、体质量、HDL-C、NUCB2、Nesfatin-1水平显著降低(P<0.05);相较于T2DM组,利格列汀组、低剂量组、高剂量组FBG水平、OGTT AUC、摄食量、摄水量、尿量、LDL-C、TG、TC、Ghrelin水平显著降低,INS水平、体质量、HDL-C、NUCB2、Nesfatin-1水平显著增加(P<0.05);相较于低剂量组,高剂量组血清FBG水平、OGTT曲线下区域面积、摄食量、摄水量、尿量、LDL-C、TG、TC、Ghrelin水平显著降低,INS水平、体质量、HDL-C、NUCB2、Nesfatin-1水平显著增加(P<0.05)。结论糖肝煎可抑制T2DM大鼠摄食,增加其体质量及血浆Nesfatin-1水平,降低Ghrelin水平。

Objective To explore the effect of Tanggan decoction on food intake and plasma NUCB2-encoded satiety and fat-influencing protein 1(Nesfatin-1)and ghrelin in in rats with type 2 diabetes mellitus(T2DM).Methods Fifty Sprague‒Dawley(SD)rats were divided into a normal group,T2DM group,linagliptin group(50 mg/kg linagliptin),low-dose group(2.8 g/kg Tanggan decoction),and high-dose group(5.6 g/kg Tanggan decoction),with 10 rats per group.The T2DM rat model was established by tail vein injection of streptozotocin(STZ)and a high-fat diet;Rats in the ligliptin,low-dose and high-dose groups were given the corresponding drugs by gavage for 4 consecutive weeks,and the normal and T2DM groups were given equal amounts of saline once/day.After the experiment,the general condition of the rats was observed,the body mass of the rats was determined,and the amount of food intake,water intake and urine output were measured.Serum fasting blood glucose(FBG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),triglycerides(TG)and total cholesterol(TC)levels were determined by an automatic biochemical analyzer;the area under the curve(AUC)of the oral glucose tolerance test(OGTT)in rats was calculated;serum insulin(INS)levels and plasma nucleonectin 2(NUCB2),ghrelin,nesfatin-1 levels were measured by enzyme-linked immunosorbent assay(ELISA);and plasma levels of nesfatin-1 and ghrelin mRNA were detected with real-time fluorescence quantitative reverse transcription-polymerase chain reaction(qRT-PCR).Results The rats in the normal group had normal stools,smooth and shiny fur,flexible movement and good mental status;while the rats in the T2DM group had loose stools,poor mental health,and yellow and rough fur.Compared with the T2DM group,the rats in the linagliptin,low-dose,and high-dose groups had different degrees of improvement in the above symptoms.Compared with the normal group,the rats in the T2DM group had significantly higher serum FBG levels,OGTT AUC,food intake,water intake,urine output,LDLC,TG,TC and ghrelin levels,and significantly lower INS levels,body weight,HDL-C,NUCB2 and nesfatin-1 levels(P<0.05).Compared with the T2DM group,the rats in the linagliptin,low-dose and high-dose groups showed significantly lower FBG levels,OGTT AUC,food intake,water intake,urine output,LDL-C,TG,TC and Ghrelin levels,and significantly higher INS levels,body weight,HDLC,NUCB2,and nesfatin-1 levels(P<0.05).Compared with the low-dose group,serum FBG levels,OGTT AUC,food intake,water intake,urine output,LDL-C,TG,TC,and ghrelin levels were significantly lower,and INS levels,body weight,HDL-C,NUCB2,and nesfatin-1 levels were significantly higher in the high-dose group(P<0.05).Conclusion Tanggan decoction can inhibit food intake,increase body weight and plasma nesfatin-1 levels,and decrease ghrelin levels in T2DM rats.