姜黄素预防高糖诱导的H9c2细胞损伤的最适浓度筛选

Selection of the Optimal Concentration of Curcumin to Prevent the Injury of the H9c2 Induced by High Glucose

为筛选出用于预防高糖诱导的H9c2细胞损伤的最适姜黄素浓度,检测不同浓度姜黄素处理下H9c2细胞的部分指标的表达,并观察细胞形态变化.结果显示,与模型组相比,姜黄素浓度为4×10^(-6),8×10^(-6)和20×10^(-6)mol/L时细胞增殖活力上升;姜黄素各组的Caspase 3和Cleaved-Caspase 3的蛋白表达均显著下调;姜黄素4×10^(-6)mol/L时,H9c2细胞形态和数量恢复,且分布均匀,排列紧密,与正常组细胞无较大差异.最终选择4×10^(-6)mol/L时的姜黄素作为预防高糖诱导的H9c2细胞损伤的最适浓度.

In order to screen out the optimal curcumin concentration for preventing H9 c2 cell damage induced by high glucose, the expression of some indexes of H9 c2 cells under different curcumin concentrations is detected, and the morphological changes of the cells are observed. The results show that cell proliferation activity increase at curcumin concentrations of 4×10^(-6) mol/L,8×10^(-6) mol/L and 20×10^(-6) mol/L compared with the model group. The protein expression of Caspase 3 and Cleaved-Caspase 3 are significantly down-regulated in curcumin groups. When curcumin is 4×10^(-6) mol/L,the morphology and number of H9 c2 cells are recover, and the distribution is uniform and the arrangement is tight with no significant difference from the control group. Curcumin at 4×10^(-6) mol/Lis finally selected as the optimal concentration for preventing H9 c2 cell damage induced by high glucose.