美国黄松和蓝粉云杉叶绿体基因组序列测定与分析

Complete chloroplast genome sequence and analysis of Pinus ponderosa P. Lawson & C. Lawson and Picea pungens Engelm.

采用高通量测序技术获得了美国黄松(Pinus ponderosa P. Lawson&C. Lawson)和蓝粉云杉(Picea pungens Engelm.)的叶绿体全基因组序列。结果显示,美国黄松和蓝粉云杉的叶绿体基因组大小分别为120 274和124 237 bp。美国黄松共注释到107个基因,其中包含70个蛋白编码基因,4个rRNA基因,33个tRNA基因;蓝粉云杉包含111个基因,其中71个蛋白编码基因,4个rRNA基因,36个tRNA基因。美国黄松和蓝粉云杉分别鉴定到18和32个简单重复序列单元。基于19个松科植物和2个外类群共有的73个叶绿体基因的系统发育分析结果,可将松科分为两大分支:第1分支包含了雪松属(Cedrus)、金钱松属(Pseudolarix)、铁杉属(Tsuga)、油杉属(Keteleeria)和冷杉属(Abies);第2分支包含了松属(Pinus)、银杉属(Cathaya)、云杉属(Picea)、落叶松属(Larix)和黄杉属(Pseudotsuga)。叶绿体基因组结构分析结果表明,松科不同属间发生了多次重排,并且是由包含trnS-GCU基因的小反向重复介导。研究结果说明叶绿体基因组序列可用于解决松科属间的系统发育关系。

In this study, the complete chloroplast genome sequences of Pinus ponderosa P. Lawson & C. Lawson and Picea pungens Engelm. were determined using next-generation sequencing technology. The genome sizes of Pinus ponderosa and Picea pungens were 120 274 bp and 124 237 bp, respectively. The chloroplast genome of P. ponderosa contained 107 genes, including 70 protein-coding genes, four ribosomal RNA(rRNA) genes, and 33 transfer RNA(tRNA) genes. The chloroplast genome of P. pungens contained 111 genes, including 71 protein-coding genes, four rRNA genes, and 36 tRNA genes. In total, 18 and 32 simple sequence repeats were identified in the Pinus ponderosa and Picea pungens chloroplast genomes, respectively. Phylogenetic analysis using 73 common chloroplast genes from 19 Pinaceae and two outgroups divided Pinaceae into two groups: the first clade included Cedrus, Pseudolarix, Tsuga, Keteleeria, and Abies;the second clade included Pinus, Cathaya,Picea,Larix, and Pseudotsuga. Analysis of chloroplast genome structure identified several rearrangements between different genera of Pinaceae, mediated by a trnS-GCU small inverted repeat sequence.