儿童末梢全血和静脉血清检测IGF-1和IGFBP-3的一致性研究

Study on the consistency of IGF-1 and IGFBP-3 of peripheral whole blood and venous serum in children

探讨末梢全血和静脉血清检测儿童胰岛素样生长因子1(IGF-1)和胰岛素样生长因子结合蛋白3(IGFBP-3)的一致性。本研究为横断面设计,纳入2022年1至4月在南京医科大学附属儿童医院儿童保健科健康体检的0~14岁儿童,同时收集末梢全血和静脉血清样本,应用化学发光免疫分析法(CLIA)分别检测两种样本的IGF-1和IGFBP-3水平。采用线性回归方程分析两种样本检测结果之间的相关性,并运用组内相关系数(ICC)评估两种样本检测结果的一致性,以及分析IGF-1和IGFBP-3随年龄的变化趋势。结果显示,共收集匹配有效样本203例(男117名,女86名)。末梢全血和静脉血清IGF-1(r=0.986,P<0.001)、IGFBP-3(r=0.974,P<0.001)相关良好,直线回归方程式分别为:IGF-1_(静脉血清)=1.047×IGF-1_(末梢全血)-6.840,IGFBP-3_(静脉血清)=0.924×IGFBP-3_(末梢全血)+0.396。按性别和年龄分层后相关性和一致性依然存在。ICC分析表明,IGF-1和IGFBP-3的单一量度ICC分别为0.983和0.967,一致性良好。男女童末梢全血和静脉血清IGF-1与IGFBP-3在各年龄组差异有统计学意义(P均<0.001),均随年龄增加而显著上升(P_(趋势)均<0.001)。综上,末梢全血和静脉血清检测IGF-1和IGFBP-3的结果具有较好的可比性,其水平可以互认。检测末梢血IGF-1和IGFBP-3在低龄儿童领域有较大的应用潜力,可为低龄儿童的营养干预、生长发育评估等提供辅助指导。

This study aimed to explore the consistency of insulin-like growth factor-1(IGF-1)and insulin-like growth factor-binding protein-3(IGFBP-3)by detecting peripheral whole blood and venous serum among children.As a cross-sectional study,children who were aged 0-14 as well as received physical examinations in the Child Healthcare Department of the Children′s Hospital of Nanjing Medical University during January 2022 to April 2022 were enrolled in this study.Meanwhile,both of peripheral whole blood and venous serum samples were collected,and the levels of IGF-1 and IGFBP-3 were assayed individually via chemiluminescence immunoassay(CLIA).Additionally,linear regression equation was used to analyze the correlation of results between two categories of samples,while Inter-class Correlation Coefficient(ICC)was used to evaluate the consistency of test results among two types of samples.The change trends of IGF-1 and IGFBP-3 with age were analyzed at the same time.A total of 203 valid matched samples were collected,including 117 boys and 86 girls.Peripheral whole blood was well correlated with serum IGF-1(r=0.986,P<0.001)and IGFBP-3(r=0.974,P<0.001),and the linear regression equation is shown as follows:(IGF-1)venous serum=1.047×(IGF-1)peripheral whole blood-6.840;(IGFBP-3)venous serum=0.924×(IGFBP-3)peripheral whole blood+0.396.The correlation and consistency were still persisted after being stratified by sex and age.ICC of IGF-1 and IGFBP-3 were 0.983 and 0.967,respectively which provided an excellent strength of agreement.The levels of IGF-1 or IGFBP-3 in boys′and girls′peripheral whole blood and serum showed significant statistical differences among various age groups(all P<0.001),and also increased significantly with age(all Ptrend<0.001).In conclusion,the results of IGF-1 and IGFBP-3 in peripheral whole blood and venous serum had positive comparability that could be mutually recognized.The detection of IGF-1 and IGFBP-3 in peripheral whole blood had great potential for young age children by providing guidance for nutritional intervention,growth and development assessment.