不同浓度氮处理毛竹水通道蛋白基因表达模式及其调控网络

Expression Pattern of Aquaporin Genes in Moso Bamboo under Different Nitrogen Concentrations and Its Regulating Network

氮和水分平衡对植物生长发育具有重要作用,在长期进化过程中植物体内形成了氮运输和水分平衡的复杂调控网络。为揭示竹子速生的内在调控机制,研究了不同浓度氮处理下毛竹根中水通道蛋白(AQP)基因的表达模式,并预测了其表达调控网络。结果表明,在不同浓度氮处理下,在毛竹根的转录本中共鉴定到30个差异表达的PeAQP s,包括14个PePIP s、8个PeTIP s、7个PeNIP s和PeSIP 2-1;随着氮浓度的提高,PeAQP s的表达模式主要分为2类:一类呈上调表达,另一类呈下调表达。在这些差异表达AQP基因的启动子中鉴定到了许多转录因子(TF)的结合元件,同时通过筛选差异表达TFs,构建了差异表达TFs和PeAQP s的共表达网络。代表性差异表达TFs和PeAQP s表达的相关性分析和共表达结果表明,这些TF可能参与调控毛竹中水分和氮的协调运输。研究结果可为深入解析毛竹快速生长的分子机制提供参考。

Nitrogen and water balance play important roles in the growth and development of plants,and a complex regulatory network of nitrogen transport and water balance has been formed in plants during their long-term evolution.The paper studies the expression patterns of aquaporin(AQP)gene in the roots of nitrogen-treated moso bamboo(Phyllostachys edulis)and predicts its expression regulation network,to reveal the internal regulation mechanism of bamboo rapid growth.The results show that a total of 30 differentially expressed PeAQP s,including 14 PePIP s,8 PeTIP s,7 PeNIP s and PeSIP 2-1,are identified in the transcripts of moso bamboo roots under different nitrogen concentrations.The expression patterns of PeAQP s are mainly divided into two types,i.e,up-regulated and down-regulated,with the increase of nitrogen concentration.A number of transcription factor(TF)binding elements are identified in the promoters of these differentially expressed PeAQP s,and a co-expression network of TFs and PeAQP s is constructed by screening for the differentially expressed TFs.The correlation analysis and co-expression results of representative differentially expressed TFs and PeAQP s indicate that these TFs could be involved in regulating the coordinated transport of water and nitrogen in moso bamboo.The results would provide a reference for further understanding the molecular mechanism of rapid growth of moso bamboo.