GmFULa基因的功能和表达特性分析

Analysis of Function and Expression Characteristics of GmFULa Gene

为阐明大豆[Glycine max(L.)Merr.]FRUITFULLa(GmFULa)基因在植物营养生长及生殖生长中的作用,利用农杆菌介导法对拟南芥进行遗传转化,对过表达GmFULa基因拟南芥的开花时间、始花期莲座叶片数及成熟期分枝数和荚果数进行分析;并以光周期敏感大豆品种自贡冬豆和光周期钝感大豆品种黑河27为试验材料,分析GmFULa基因在长日照和短日照条件下全生育期的表达规律;同时在短日照条件下分析GmFULa基因在自贡冬豆不同组织中的表达特性及昼夜表达规律。结果表明,过表达GmFULa基因增加了拟南芥莲座叶片数、分枝数和荚果数,并可能使拟南芥开花提前。GmFULa基因在生殖生长期的表达量远高于营养生长期;在荚果中的表达量最高,远远高于其他组织;呈现出昼高夜低的昼夜表达节律,并受光信号诱导。综上,GmFULa基因对植物的营养生长和生殖生长均有影响,并在荚果发育中发挥重要作用。

To clarify the function of GmFULa[Glycine max(L.)Merr.FRUITFULLa]gene in plant vegetative and reproductive growth,genetic transformation of Arabidopsis thaliana was performed by Agrobacterium mediated floral dip method,and phenotype(flowering time,number of rosette leaves at the beginning of flowering,number of branches and pods at maturity)of Arabidopsis thaliana overexpressing GmFULa gene was analyzed.Meanwhile,the soybean varieties Zigongdongdou(photoperiod sensitive variety)and Heihe 27(photoperiod insensitive variety)were used as materials to analyze the expression of GmFULa gene during the whole growth period under long day and short day conditions.Finally,the tissue expression specificity and circadian rhythm of GmFULa gene were analyzed in Zigongdongdou under short day condition.The results showed that overexpression of GmFULa gene increased the rosette leaves,branches and pods number,and might make the flowering of Arabidopsis thaliana advance.Gene expression analysis showed that the expression level of GmFULa gene at reproductive growth stage was higher than that at vegetative growth stage.The expression level of GmFULa gene in pods was the highest,far higher than that in other tissues.GmFULa gene showed a diurnal rhythm of high expression level at day and low expression level at night,and was induced by optical signal.In conclusion,GmFULa gene affects plant vegetative and reproductive growth,and plays an important role in pod development.