整合素αvβ6在头颈部鳞状细胞癌组织中的表达情况及意义

Expression and significance of integrin αvβ6 in head and neck squamous cell carcinoma

目的 分析整合素αvβ6在人头颈部鳞状细胞癌(head and neck squamous carcinoma,HNSCC)组织以及癌旁组织中的表达差异,探讨其与患者临床病理特征的关系。方法 收集2021年3~7月于武汉大学人民医院行根治性切除手术的65例HNSCC患者临床病例资料,通过免疫组织化学染色、免疫组织荧光染色及流式细胞术检测整合素αvβ6在HNSCC患者癌组织及癌旁组织中表达情况。结果 免疫组织化学染色结果显示,整合素αvβ6在HNSCC组织及癌旁组织中表达的平均光密度值分别为0.1317±0.0007和0.0870±0.0004,差异有统计学意义(t=22.9717,P<0.05)。且整合素αvβ6表达水平在不同肿瘤分化程度、TNM分期、浸润深度、有无淋巴结转移中存在差异(P<0.05)。免疫组织荧光染色结果显示,整合素αvβ6阳性表达主要分布在HNSCC癌细胞的胞质和胞膜中。流式细胞术结果显示,HNSCC癌组织中整合素αvβ6阳性表达率为78.4%,相应癌旁组织中阳性表达率为15.7%。结论 整合素αvβ6主要分布于HNSCC癌细胞的胞质和胞膜中,在HNSCC癌组织中的表达水平明显高于癌旁组织,且与其临床特征紧密相关,可能参与HNSCC的侵袭和转移。整合素αvβ6有望作为HNSCC的生物标志物,作为HNSCC预后预测指标及治疗的潜在靶点。

OBJECTIVE To observe the expression of integrin αvβ6 in human head and neck squamous cell carcinoma(HNSCC) tissues and adjacent tissues,and to explore its relationship to clinicopathological features.METHODSThe clinical data of HNSCC patients who underwent radical resection in Renmin Hospital of Wuhan University from March to July 2021 were collected.Immunohistochemical staining,immunohistofluorescence staining and flow cytometry were used to detect the expression of integrin αvβ6 in cancer tissues and adjacent tissues of 65 HNSCC patients.RESULTSImmunohistochemical staining showed that the average optical density value of integrin αvβ6 in HNSCC tissues and adjacent tissues was 0.1317±0.0007 and 0.0870±0.0004,respectively,and the difference was statistically significant(t=22.9717,P<0.05).There were significant differences in the expression level of integrin αvβ6 in different tumor differentiation degree,TNM stage,depth of invasion and lymph node metastasis (P<0.05).Immunofluorescence staining showed that the positive expression of integrin αvβ6 was mainly distributed in the cytoplasm and membrane of HNSCC cells.The results of flow cytometry showed that the positive expression rate of integrin αvβ6 was 78.4% in HNSCC tissues and 15.7% in adjacent tissues.CONCLUSION Integrin αvβ6 is mainly distributed in the cytoplasm and membrane of HNSCC cells.The expression level of integrin αVβ6 in HNSCC tissues is significantly higher than that in adjacent tissues,and it is closely related to its clinical characteristics,which may be involved in the invasion and metastasis of HNSCC.Integrin αvβ6 is expected to be a biomarker of HNSCC,a prognostic indicator and a potential therapeutic target for HNSCC.