含铜硅酸钙纳米棒复合水凝胶用于肿瘤治疗和皮肤伤口愈合性能研究

Copper-incorporated Calcium Silicate Nanorods Composite Hydrogels for Tumor Therapy and Skin Wound Healing

为了清除皮肤肿瘤手术切除后的残余肿瘤细胞并促进皮肤伤口愈合,开发一种具有肿瘤治疗和促进皮肤伤口愈合功能的水凝胶具有重要意义。本研究以水合硅酸钙纳米线为基体材料,以NaCl和KCl为熔盐介质,CuSO_(4)·5H_(2)O为铜源,采用熔盐法制备了含铜硅酸钙(Cu-CS)纳米棒,并将其复合到海藻酸钠水凝胶得到Cu-CS纳米棒复合水凝胶(Cu-CS/SA)。实验结果表明,随着铜盐添加量增大和熔盐处理温度升高,Cu-CS纳米棒的Cu含量逐渐上升,但其催化过氧化氢(H_(2)O_(2))生成羟基自由基(·OH)的性能呈现先升高后下降的趋势;在3%铜盐添加量和熔盐处理温度700℃条件下所制备的3Cu-CS纳米棒具有最佳的催化性能,Cu元素均匀地分布在纳米棒表面,其价态为+2价,且Cu元素的含量极低,仅为0.61%。细胞实验发现Cu-CS纳米棒含量不超过20%的复合水凝胶具有良好的生物相容性,并且Cu-CS/SA水凝胶在模拟肿瘤微环境条件下能催化H_(2)O_(2)生成高细胞毒性的·OH,进而实现化学动力学治疗肿瘤的效果,同时还能促进血管内皮细胞和成纤维细胞的增殖和迁移。因此,Cu-CS纳米棒复合水凝胶有望用于皮肤肿瘤术后治疗。

Developing a hydrogel with tumor therapy and skin wound healing is of great significance for eliminating residual tumor cells and promoting skin wound healing after surgical resection of skin cancers.Here,copperincorporated calcium silicate(Cu-CS)nanorods were prepared by a molten salt method with calcium hydrate silicate as matrix,NaCl and KCl as molten salt,and CuSO_(4)·5H_(2)O as Cu source,and then incorporated into sodium alginate(SA)hydrogel to achieve Cu-CS/SA composite hydrogel.The results showed that Cu content of Cu-CS nanorods increased with the increase of the Cu source addition and the treatment temperature,but their catalytic activity for producing hydroxy radical(·OH)from H_(2)O_(2) exhibited the trend of increasing and then decreasing.Nanorods,prepared with 3%copper source(3Cu-CS)at 700℃,displayed the best catalytic performance.Cu with+2 valence state could be uniformly distributed on the surface of Cu-CS nanorods,with Cu content as low as 0.61%.Importantly,Cu-CS/SA hydrogel with Cu-CS nanorods less than 20%were biocompatible and could catalyze H_(2)O_(2) to produce cytotoxic·OH in a simulated tumor microenvironment,exhibiting outstanding chemodynamic effect.Furthermore,Cu-CS/SA hydrogel could promote proliferation and migration of human umbilicle vein endothelial cells and human dermal fibroblast.Therefore,Cu-CS/SA hydrogel is a promising material for applying in tumor therapy and skin wound healing.