肝癌患者WNT通路分子C-myc和β-catenin表达水平与恶性程度的关系分析

Relationship between Wnt pathway molecular C-myc andβ-catenin expression and malignancy in patients with liver cancer

目的探究WNT通路分子C-myc癌基因(C-myc)和β-连环蛋白(β-catenin)与原发性肝癌患者肝癌的恶性程度、临床分级、转移及患者的预后关系。方法选择2019年1月至2022年1月在邯郸市中心医院收治的肝癌患者84例作为观察组,同时收集肝硬化患者和乙型肝炎患者共60例作为对照组,收集患者病理组织标本,采用免疫组化法检测两组患者组织中C-myc和β-catenin的表达水平,分析C-myc、β-catenin与原发性肝癌患者临床病理特征的关系及患者预后的关系。结果观察组C-myc和β-catenin的阳性率均高于对照组,差异有统计学意义(P<0.01);高甲胎蛋白(AFP)水平、无包膜或包膜不完整、低中分化肿瘤、临床分期为Ⅲ~Ⅳ期、病理类型为胆管上皮癌、混合型肝癌及出现肝内外转移的肝癌患者C-myc和β-catenin阳性率较高,差异有统计学意义(P<0.01);肝癌患者1、2、3年生存率分别为71.31%、52.35%、36.20%,随访3年存活患者的C-myc和β-catenin阳性率低于1年及2年存活患者,差异有统计学意义(P<0.01)。结论组织中C-myc和β-catenin阳性率与原发性肝癌患者AFP水平、肿瘤包膜情况、分化程度、临床分期、组织学类型及预后具有一定关系。

Objective To explore the relationship between WNT pathway molecule C-myc oncogene(C-myc)andβ-catenin and the malignant degree,clinical grade,metastasis and prognosis of patients with primary liver cancer.Methods A total of 84 patients with liver cancer treated in Handan Central Hospital from January 2018 to January 2019 were selected as the observation group,and 60 patients with liver cirrhosis and hepatitis B were collected as the control group.The pathological tissue samples were collected,and the expression levels of C-myc andβ-catenin in the tissues of the two groups were detected by immunohistochemistry.Relationship between the expression levels of C-mycβ-catenin and clinical features and prognosis of patients with primary liver cancer were analyzed.Results The positive rates of C-myc andβ-catenin in the observation group were higher than those in the control group,and the differences were statistically significant(P<0.01).The positive rates of C-myc andβ-catenin were higher in patients with high alpha fetoprotein(AFP)level,no capsule or incomplete capsule,low and moderately differentiated tumor,clinical stage ofⅢ—Ⅳ,pathological type of cholangioepithelial carcinoma,mixed liver cancer and liver cancer with extrahepatic and extrahepatic metastasis,and the differences were statistically significant(P<0.01).The 1-year,2-year and 3-year survival rates of patients with liver cancer were 71.31%,52.35%and 36.20%,respectively.The positive rates of C-myc andβ-catenin in patients who survived for 3 years were lower than those in patients who survived for 1 and 2 years,and the differences were statistically significant(P<0.01).Conclusion The positive rates of C-myc andβ-catenin had some relationship with AFP level,tumor envelope,degree of differentiation,clinical stage,histological type and prognosis in patients with primary liver cancer.