植物乳杆菌胞外多糖的单糖组成及抗氧化活性

Monosaccharide Composition and Antioxidant Activity of Exopolysaccharides of Lactobacillus plantarum

分析植物乳杆菌胞外多糖的单糖组成,同时对其抗氧化活性进行研究。以植物乳杆菌发酵液为原料,通过水提醇沉方法得到植物乳杆菌粗多糖(DZ),经DEAE-52纤维素柱分离纯化收集得到DZ-1与DZ-22种多糖组分,采用气相色谱法对DZ-1与DZ-2中单糖组成进行分析,测定其1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、羟自由基和2,2’-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)(2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid),ABTS)阳离子自由基清除率,对2种多糖组分的抗氧化活性进行研究。结果表明:DZ-1为仅含葡萄糖的均多糖,DZ-2则为由阿拉伯糖与半乳糖组成的杂多糖,其单糖组成及百分比含量比值为阿拉伯糖∶半乳糖=46.03∶53.89;抗氧化实验结果表明,DZ-1与DZ-2均具有一定的DPPH自由基、羟自由基和ABTS阳离子自由基清除能力。

The monosaccharide composition and antioxidant activity of extracellular polysaccharides from Lactobacillus plantarum were analyzed.Crude polysaccharides were obtained from the fermentation broth of Lactobacillus plantarum by water extraction and alcohol precipitation and were fractionated into two fractions(DZ-1 and DZ-2)by DEAE-52 cellulose column chromatography.The monosaccharide components of DZ-1 and DZ-2 were analyzed by gas chromatography,and their antioxidant activity was tested using 1,1-diphenyl-2-picrylhy drazyl(DPPH),hydroxyl radical,and 2,2’-diazo bis-3-ethyl benzothiazoline-6-sulfonic acid(ABTS)cation radical scavenging assays.DZ-1 was a homopolysaccharide composed of only glucose units,while DZ-2 was a heteropolysaccharide composed of arabinose and galactose with a content ratio of 46.03:53.89.Both DZ-1 and DZ-2 had the ability to scavenge DPPH,hydroxyl and ABTS cation radicals.