玉米DUF1685基因家族的鉴定和进化分析

Identification and Evolutionary Analysis of Maize DUF1685 Gene Family

本研究共鉴定出16个单、双子叶植物和卷柏的211个DUF1685基因家族成员。蛋白理化性质分析表明,211个DUF1685s基因的氨基酸序列长度为83~1071 aa,分子量为9.2~116.3kDa,理论等电点为3.66~11.90。系统发育进化分析表明,DUF1685基因家族被划分了3个亚家族(Class I、Class II和Class III),Class I和Class II亚家族产生A1和A2、B1和B2的事件发生在单、双子叶植物分化后,同时A1和B2分支(成员均为双子叶植物基因)出现了基因扩张现象。保守基序和基因结构分析结果表明,同一亚家族成员之间保守基序相似,211个DUF1685基因家族成员中大部分含有1个内含子,少部分基因含有2个及以上内含子或者不含内含子。选择压分析表明,DUF1685基因家族在进化过程中相关位点受到正向选择,这可能是导致部分基因发生功能变化的原因。共线性分析表明,片段复制事件可能是玉米DUF1685基因扩增和进化的潜在驱动力。基因启动子区域顺式元件分析结果表明,玉米DUF1685基因启动子区域含有光响应元件、ABA和非生物胁迫相关的顺式作用元件,推测这些基因可能参与玉米对非生物胁迫的响应。转录组数据分析表明,玉米DUF1685基因与特定组织(成熟花粉)的生长发育相关。本研究从多方面探究了玉米DUF1685基因家族的功能和进化情况,以期为今后深入研究玉米DUF1685基因生物学功能和进化关系提供理论参考。

A total of 211 DUF1685 gene family members in 16 monocotyledonous and dicotyledon and Selaginella moellendorffii were identified in this study. Analysis of protein physicochemical properties showed that the amino acid sequences of 211 DUF1685 genes ranged in length from 83 to 1071 aa, molecular weights from 9.2 to 116.3 kDa, and theoretical isoelectric points from 3.66 to 11.90. Phylogenetic evolution analysis showed that the DUF1685 gene family were divided into three subfamilies(Class I, Class II and Class III). The events of A1 and A2, B1 and B2 in the Class I and Class II subfamilies occurred after the differentiation of monocotyledonous plants, and A1 and B2 branches(members were all dicotyledonous genes) had gene expansion phenomenon. The results of conserved motifs and gene structure analysis showed that the conserved motifs among members of the same subfamily were similar. Most of the 211DUF1685 family genes contained one intron, and a few genes contained two or more introns or no introns. Selection pressure analysis showed that the related loci of DUF1685 family genes were positively selected during the evolution process, which may be the reason for the functional changes of some genes. Collinearity analysis indicated that fragment duplication events may be a potential driver of DUF1685 gene amplification and evolution in the maize. The results of cis-element analysis in the gene promoter region showed that the promoter region of maize DUF1685 gene contained light-responsive elements, ABA and cis-acting elements related to abiotic stress. It was speculated that the genes may be involved in the response of maize to abiotic stress. Transcriptome data analysis revealed that the maize DUF1685 gene was associated with the growth and development of specific tissues(mature pollen). This study explored the function and evolution of the maize DUF1685 gene family in various aspects to provide a theoretical reference for the in-depth study of the biological function and evolutionary relationship of the maize DUF1685 gene in the future.