丹参萌发期抗旱性鉴定及抗旱指标筛选

Drought resistance identification and drought resistance index selection of Salvia miltiorrhiza during germination

以5个丹参栽培类型为材料,用不同浓度(0%、3%、6%、9%、12%)的PEG-6000在萌发期模拟干旱胁迫处理,测定发芽势、发芽率、子叶长、子叶宽、胚芽长、胚根长等指标,采用主成分分析和隶属函数分析进行丹参萌发期的抗旱性鉴定及抗旱指标筛选。结果表明:高浓度PEG对种子萌发产生明显的抑制作用,低浓度(3%PEG-6000)处理对丹参种子的生长发育具有促进作用,5个丹参栽培类型中豫丹参VD发芽率(0%PEG-6000)和发芽势(3%PEG-6000)最高,分别为56.50%、55.50%,豫丹参VC、豫丹参VD和豫丹参VE在12%PEG-6000下发芽率、发芽势为0%;随着PEG胁迫程度的增大,发芽势、发芽率、胚芽长、胚根长、子叶长、子叶宽、十芽重、萌发指数和活力指数总体表现出先增大后减小的趋势。对各指标进行主成分分析,前2个主成分能够概括9个指标82.635%的数据信息,对在前2个主成分中特征向量较大的8个综合指标的相对值进行因子分析,可提取出2个公因子,子叶长、子叶宽、活力指数、十芽重和萌发指数在2个公因子上有较大载荷。9%PEG-6000是较适宜的抗旱性鉴定浓度。根据综合隶属函数值可得出5个供试材料抗旱性强弱顺序:豫丹参VE>豫丹参VC>豫丹参VB>豫丹参VD>豫丹参VA,其综合隶属函数值分别为:0.071、0.068、0.054、0.043、0.028,豫丹参VE为抗旱能力较强的种质,豫丹参VC抗旱能力次之,豫丹参VD和豫丹参VA抗旱性较弱;子叶长、子叶宽、活力指数、十芽重和萌发指数可以作为抗旱鉴定指标。

To compare the drought resistance of different Salvia miltiorrhiza cultivar types and select drought-resistant varieties,five Salvia miltirrohiza cultivar types were treated with different concentrations(0%,3%,6%,9%,12%)of PEG-6000 at germination stage to simulate drought stress.The germination potential,germination rate,cotyledon length,cotyledon width,radicle length and radicle length were measured.Principal component analysis and membership function analysis were used for identification of drought resistance and selection of drought resistance indexes at the germination stage of Salvia miltiorrhiza.The results showed that high concentration of PEG had an obvious inhibitory effect on seed germination,and low concentration(3%PEG-6000)treatment had a promoting effect on the growth and development of Salvia miltiorrhiza seeds.Among the five cultivation types of Salvia miltiorrhiza,Yu dan shen VD had the highest germination rate(0%PEG-6000)and germination potential(3%PEG-6000),which were 56.50%and 55.50%,respectively.Yu dan shen VC,Yu dan shen VD and Yu dan shen VE had the lowest germination rate under 12%PEG-6000,which was only 0%.With the increase of PEG stress,the germination potential,germination rate,germ length,radicle length,cotyledon length,cotyledon width,ten bud weight,germination index and vigor index showed a trend of first increasing and then decreasing.Principal component analysis was carried out for each indicator.The first two principal components summarized 82.635%of the data information of nine indicators.Factor analysis was carried out for the relative values of eight comprehensive indicators with large feature vectors in the first two principal components.The 9%PEG-6000 concentration was a more suitable one for drought resistance identification.According to the comprehensive membership function value,the order of drought resistance of five tested materials was obtained:Yu Danshen VE>Yu Danshen VC>Yu Danshen VB>Yu Danshen VD>Yu Danshen VA.The comprehensive membership function values were 0.071,0.068,0.054,0.043,0.028.Yu dan shen VE had strong drought resistance.Yu dan shen VC had the second strongest drought resistance.Yu dan shen VD and Yu dan shen VA had weaker drought resistance.Cotyledon length,cotyledon width,vitality index,ten bud weight and germination index should be used as drought resistance identification indexes.