不同种子处理方法下长枝木霉T6菌株对小油菜促生作用及生理特性的影响

Effects of Trichoderma longibrachiatum T6 on growth promotion and physiological characteristics of Brassica chinensis under different seed treatment methods

为了明确长枝木霉T6菌株(Trichoderma longibrachiatm)对小油菜(Brassica chinensis)促生效果及生理特性的影响,通过水琼脂培养法、浸泡法和灌根法进行种子处理,并测定了长枝木霉T6菌株及其孢子悬浮液和发酵滤液处理后小油菜种子活性、根系发育及幼苗叶绿素和可溶性蛋白含量的变化情况。结果表明:与对照相比,采用水琼脂培养法,接种长枝木霉T6菌株后小油菜种子发芽率、发芽指数、胚根长度、胚芽长度、幼苗鲜重和干重相对增长率分别为2.51%、20.55%、70.79%、21.98%、32.00%和30.00%,幼苗根系总投影面积、总根表面积、平均根系直径、总根体积和根尖数相对增长率分别为58.49%、58.79%、18.18%、100.00%和77.88%;采用种子浸泡法,浓度为1.0×10^(7) cfu·mL^(-1)长枝木霉T6菌株孢子悬浮液处理后显著提高了小油菜种子发芽率、发芽指数、胚根长度、胚芽长度、幼苗鲜重和干重,其相对增长率分别为2.06%、19.60%、82.21%、43.36%、31.58%和40.00%,根系总投影面积、总根表面积、平均根系直径、总根体积和根尖数相对增长率分别为44.19%、45.52%、13.04%、100.00%和28.57%;温室盆栽试验表明,长枝木霉T6菌株发酵滤液能够显著提高小油菜幼苗叶绿素和可溶性蛋白含量,与对照相比,处理后其叶绿素和可溶性蛋白含量相对增长率分别为31.95%和11.40%。研究表明,水琼脂培养和种子浸泡处理下长枝木霉T6菌株对小油菜具有显著促生作用。

In this study,the effects of Trichoderma longibrachiatum T6 strain and its spore suspension and fermentation filtrate on the changes of seed activity,root development,leaf chlorophyll content and soluble protein content of Brassica chinensis were evaluated using different seed treatment methods of water-agar culture,soaking and root irrigation.The results showed that the relative growth rates of seed germination rate,germination index,radicle length,plumule length,fresh weight and dry weight were increased by 2.51%,20.55%,70.79%,21.98%,32.00%and 30.00%after treating with T.longibrachiatum T6 by the method of water-agar culture,respectively.The relative growth rates of total projected root area,total root surface area,average root diameter,total root volume and root tip number were increased by 58.49%,58.79%,18.18%,100.00%and 77.88%,respectively.Using the seed soaking method,the T.longibrachiatum T6 spore suspension(1.0×10^(7) cfu·mL^(-1))significantly increased the seed germination rates,germination index,radicle length,plumule length,fresh weight and dry weight and the relative growth rates by 2.06%,19.60%,82.21%,43.36%,31.58%and 40.00%,respectively.The relative growth rates of total projected root area,total root surface area,average root diameter,total root volume and root tip number were increased by 44.19%,45.52%,13.04%,100.00%and 28.57%,respectively.Greenhouse experiment showed that the fermentation filtrate of T.longibrachiatum T6 significantly increased the chlorophyll and soluble protein contents of B.chinensis seedlings.Compared with the control,the relative growth rates of chlorophyll and soluble protein contents were increased by 31.95%and 11.40%after treating with the fermentation filtrate of T.longibrachiatum T6,respectively.Therefore,T.longibrachiatum T6 strain exhibited significantly promoting effect on B.chinensis growth by the application of water agar culture,soaking and root irrigation methods.