正交试验法优化灵芝菌丝体γ-氨基丁酸提取工艺

Optimization of the Extraction Process ofγ-aminobutyric Acid from Ganoderma lucidum Mycelia by Orthogonal Experiment

为获得灵芝菌丝体中GABA的最优提取工艺,以两阶段培养的灵芝菌丝体为研究对象,采用水提法浸提灵芝菌丝体的GABA,通过单因素和正交试验确定最佳的工艺条件。结果表明:水提法提取灵芝菌丝体中GABA,影响最大的因素是浸提时间,其次是超声功率、料液比、浸提温度,经正交试验得到最优提取工艺为:浸提时间80 min、超声功率300 W、浸提温度80℃、料液比1∶30,在此条件下GABA含量达到1.700 mg·g^(-1)。

In order to obtain the optimal extraction process of GABA from Ganoderma lucidum mycelia,the two-stage cultivated Ganoderma lucidum mycelia were taken as the research object,and the GABA from Ganoderma lucidum mycelia was extracted through the water extraction method.The optimal process conditions were determined by using the single factor and orthogonal experiments.The results showed that the most important factor affecting the extraction of GABA from Ganoderma lucidum mycelia by water extraction was the extraction time,followed by the ultrasonic power,the ratio of material to liquid and the extraction temperature.The optimum extraction process was obtained by orthogonal test:the extraction time of 80 min,the ultrasonic power of 300 W,the extraction temperature of 80℃,the ratio of material to liquid of 1∶30.Under these conditions,the content of GABA reached 1.700 mg·g^(-1).