QuEChERS结合GC-MS/MS测定辛辣蔬菜中18种有机磷农药

Determination of 18 Kinds of Organophosphorus Pesticides in Pungent Vegetables by QuEChERS Combined with GC-MS/MS

建立QuEChERS结合气相色谱-串联质谱法测定7种辛辣蔬菜中18种有机磷农药的分析方法。以7种辛辣蔬菜为材料,试验选择提取剂、质谱条件、净化剂等条件,从而确定7种辛辣蔬菜中18种有机磷农药提取检测方法。结果表明:以乙腈作为提取剂,PSA用量为100 mg,GCB用量为10 mg时,18种有机磷农药在0.05~2.00 mg·L^(-1)范围内线性良好(r^(2)>0.99);在0.05、0.10和0.50 mg·kg^(-1)3个添加水平的平均回收率介于61.25%~119.68%,相对标准偏差RSD(n=6)均小于5.0%,定量限(信噪比≥10)小于0.01 mg·kg^(-1)。该方法快速、高效、简便、经济、可靠,可以用于测定7种辛辣蔬菜中18种有机磷农药残留。

A method was developed for the determination of 18 kinds of organophosphorus pesticides in 7 kinds of pungent vegetables by QuEChERS combined with gas chromatography-tandem mass spectrometry.By taking 7 kinds of pungent vegetables as the materials,the conditions of extractant,mass spectrometry and purifying agent were selected to determine the extraction and detection methods for 18 kinds of organophosphorus pesticides in 7 kinds of pungent vegetables.The results showed that 18 kinds of organophosphorus pesticides had good linearity in the range of 0.05-2.00 mg·L^(-1)(r^(2)>0.99)when the acetonitrile was used as the extractant,and the dosage of PSA&GCB was 100 mg and 10 mg,respectively.The average recoveries at three supplemental levels of 0.05,0.10 and 0.50 mg·kg^(-1)were in the range of 61.25%-119.68%.The relative standard deviations(RSD,n=6)were less than 5.0%,and the limits of quantitation(signal-to-noise ratio≥10)were less than 0.01 mg·kg^(-1).The method was rapid,efficient,simple,economical and reliable,which could be used to determine 18 kinds of organophosphorus pesticide residues in 7 kinds of pungent vegetables.