IL-6基因多态性与环境因素对原因不明复发性流产的交互影响

Interaction effect between IL-6 polymorphisms and environmental factors on risk of unexplained recurrent spontaneous abortion

目的:探讨IL-6基因rs1800796位点单核苷酸多态性(SNP)与原因不明复发性流产(URSA)遗传易感性的关系,并分析其与相关环境因素的交互效应。方法:收集2019年1月至2020年8月在甘肃省妇幼保健院就诊的150例URSA患者为病例组,选取同期在该医院体检的150例健康育龄期女性为对照组。采用SNaPshot多重SNP分型技术检测IL-6基因相关位点SNP。以Logistics回归模型计算相关比值比(OR)及95%可信区间(CI)。结果:显性遗传模型分析显示,携带G等位基因女性发生URSA的风险增加2.03倍(OR=2.03,95%CI:1.18~3.47),发生3次及以上URSA的风险增加2.15倍(OR=2.15,95%CI:1.17~3.61)。携带GC+GG基因型分别与吸烟(包括主动和被动)、接触环境高危因素及从事重体力劳动存在正向相乘的交互作用(OR=1.96,95%CI:1.15~3.35;OR=2.18,95%CI:1.29~3.68;OR=3.27,95%CI:1.98~5.40)。结论:IL-6基因rs1800796位点可能是URSA的易感基因,且分别与吸烟(包括主动和被动)、接触环境高危因素及从事重体力劳动间存在正向协同的交互作用。

Objective:To investigate the relationship between rs1800796 single nucleotide polymorphism(SNP)of IL-6 gene and genetic susceptibility to unexplained recurrent abortion(URSA),and analyze the interaction effect with related environmental factors.Methods:A total of 150 URSA patients admitted to the Maternal and Child Health Hospital of Gansu Province from January2019 to August 2020 were enrolled as the case group,150 healthy women of childbearing age who underwent physical examination in this hospital during the same period were selected as the control group.SNPs at IL-6 gene related loci were detected by SNaPshot multiple SNP typing.Correlation odds ratio(OR)and 95%confidence interval(CI)were calculated by Logistics Regression Model.Results:Analysis of dominant genetic model showed that women with G allele had 2.03 times increased risk of developing URSA(OR=2.03,95%CI:1.18~3.47),the risk of three or more URSA episodes increased by 2.15 times(OR=2.15,95%CI:1.17~3.61).The carrier of GC+GG genotype had a positive and multiplying interaction with smoking(active and passive),exposure to environmental risk factors and heavy manual labor,respectively(OR=1.96,95%CI:1.15~3.35;OR=2.18,95%CI:1.29~3.68;OR=3.27,95%CI:1.98~5.40).Conclusion:The rs800796 site of IL-6 gene may be a susceptibility gene for URSA,which had positive synergistic interactions with smoking(both active and passive),exposure to environmental risk factors,and engaging in heavy physical labor.