绿藻硫酸多糖UCS2的结构及抗凝血活性研究

Structure characterization and anticoagulant activity of the green algal sulfated polysaccharide UCS2

目的对绿藻硫酸多糖UCS2的结构及抗凝血活性进行研究。方法通过冷水提取、强阴离子交换色谱和凝胶渗透色谱,从绿藻花石莼中提取分离得到硫酸多糖UCS2;采用高效凝胶渗透色谱、高效液相色谱、红外光谱和气质联用色谱对多糖UCS2的结构进行表征;通过活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、凝血酶原时间(PT)对多糖UCS2体外抗凝血活性进行研究。结果绿藻多糖UCS2相对分子质量为3.955×10^(4),硫酸根和糖醛酸质量分数分别为19.74%和18.66%;UCS2主要由鼠李糖组成,含有少量葡糖醛酸和木糖。糖链中含有→3,4)-α-L-Rhap-(1→,→4)-α-L-Rhap-(1→,→4)-β-D-Xylp-(1→和→4)-β-D-Glc Ap-(1→,硫酸基位于→4)-α-L-Rhap-(1→的C-3位。多糖UCS2对APTT有显著延长作用,具有较高的抗凝活性。结论绿藻多糖UCS2是1种具有抗凝活性的葡萄糖醛酸-木糖-鼠李糖型硫酸多糖。

Objective To investigate the structural characterization and anticoagulant activity of the green algal sulfated polysaccharide UCS2.Methods Sulfated polysaccharide(UCS2)was extracted from the green alga Ulva conglobata by water at room temperature,and further purified by strong anion exchange chromatography and gel permeation chromatography.The structure of the sulfated polysaccharide was characterized by high performance gel permeation chromatography(HPGPC),high performance liquid,Fourier-transform infrared spectroscopy and gas chromatography-mass spectrometry.The anticoagulant activity of polysaccharide was determined by activated partial thromboplastin time(APTT),thrombin time and prothrombin time in vitro.Results The average molecular weight of the polysaccharide UCS2 was 3.955×10^(4).The contents of sulfate eater and uronic acid were 19.74%and 18.66%,respectively.UCS2 was mainly composed of rhamnose with small amounts of glucouronic acid and xylose.The polysaccharide chain of UCS2 consisted of→3,4)-α-LRhap-(1→,→4)-α-L-Rhap-(1→,→4)-β-D-Xylp-(1→and→4)-β-D-GlcAp-(1→units.The sulfate eater was located at C-3 of→4)-α-L-Rhap-(1→unit.The polysaccharide UCS2 could significantly prolong APTT in vitro,and exhibited a strong anticoagulant activity.Conclusion The polysaccharide UCS2 was a sulfated glucurono-xylo-rhamnan with anticoagulant activity.