结合Attention U-Net与瓶颈检测的肺部细胞图像分割方法

Lung cell image segmentation method combining Attention U-Net and bottleneck detection

肺部病理图像具有边界模糊、细胞重叠交织等特点,为了解决细胞分割问题,提出结合Attention U-Net与瓶颈检测的肺部细胞图像分割方法。首先对采集到的图像进行双边滤波和拉普拉斯锐化处理,在去除噪声的同时突出细胞边缘细节,加大目标物与背景的对比;然后对Attention U-Net进行训练,利用训练的模型对病理图像进行分割,得到细胞区域;在模型分割结果的基础上,以面积、周长、圆度为筛选条件建立判别模型,区分单个细胞和重叠细胞;对细胞重叠区域采用瓶颈检测方法确定分离点,采用椭圆拟合方法进行边界修正,得到最终分割结果。实验结果表明,该方法能够对复杂的肺部细胞病理图像进行分割(包括单个细胞与重叠细胞),取得了较好的分割结果。

Lung pathological images are characterized by fuzzy boundary and overlapping and interweining cells.In order to solve the problem of cell segmentation,a lung cell image segmentation method combining Attention U-Net and bottleneck detection was proposed.Firstly,bilateral filtering and Laplacian sharpening were performed on the collected images to highlight the details of cell edges and increase the contrast between the target and the background while removing the noise.Then the Attention U-Net was trained,and the pathological images were segmented using the trained model to obtain the cell regions.Based on the segmentation results of the model,the discriminant model was established with area,circumference and roundness as screening conditions to distinguish single cell from overlapping cells.The bottleneck detection method was used to determine the separation point in the overlapping region of cells,and the ellipse fitting method was used to modify the boundary,and the final segmentation result was obtained.Experimental results show that this method can segment complex lung cell pathological images(including single cell and overlapping cells)and achieve good segmentation results.