德国鸢尾IgSVP基因的克隆及其表达分析

Cloning and Expression Analysis of IgSVP Gene from Iris germanica L.

本研究以德国鸢尾‘2010200’为试验材料,通过同源重组法克隆获得成花抑制蛋白SVP (short vegetative phase)家族关键基因IgSVP,其全长开放阅读框(ORF)为675 bp,编码225个氨基酸。根据氨基酸序列保守性分析显示,该基因含有一个保守的MADS-box和K-box结构域。氨基酸序列比对及系统进化树分析表明,Ig SVP蛋白与油棕EgSVP的亲缘关系最近。RT-qPCR分析表明,IgSVP具有组织表达特异性,在德国鸢尾的叶中表达量较高。在花发育不同时期,IgSVP表达量呈现先增长后下降的趋势,在蕾期Ⅱ表达量最高,盛花期表达量最低。根据以上结果推测,IgSVP基因可能参与德国鸢尾的花发育调控。

IgSVP, a key gene of SVP(short vegetative phase) family, was cloned from Iris germanica ’2010200’ by homologous recombination method using, and its open reading frame(ORF) was 675 bp, encoding 225 amino acids.According to the conserved amino acid sequence analysis, this gene contains a conserved MADS-box and K-box domains. Amino acid sequence alignment and phylogenetic analysis showed that Ig SVP protein was the most closely related to EgSVP. The tissue specificity expression of IgSVP was identified by real-time quantification PCR(RT-qPCR), and the results showed that IgSVP was highly expressed in leaves. At different stages of flower development, the expression of IgSVP firstly increased and then decreased, which was the highest in bud Ⅱ and the lowest in the full bloom. These results suggested that IgSVP may be involved in the regulation of flower development of I. germanica.