马铃薯资源糖苷生物碱含量评价及合成相关基因表达分析

Evaluation of Steroidal Glycoalkaloids and Analysis of Related Gene Expression in Potato Resources

检测11个马铃薯资源叶片和薯皮中糖苷生物碱(Steroidalglycoalkaloids,SGA)含量,筛选低SGA含量的马铃薯资源,并对下寨65、LZ111、Q072625、陇薯7号、青薯9号和青薯11号薯皮中SGA合成途径中相关基因的表达进行分析,以期明确马铃薯SGA生物合成关键基因,为创制低SGA马铃薯资源提供新思路。结果表明:(1)11个马铃薯资源叶片中SGA含量范围为0.08600~1.58066 mg·g^(-1)FW;(2)11个马铃薯资源薯皮中SGA含量范围为0.01415~0.18450 mg·g^(-1)FW,薯皮总SGA含量均小于0.20 mg·g^(-1)FW(安全范围);(3)6个马铃薯资源块茎中SGA合成途径相关基因表达量分析表明,StSQS1、StCAS、StSSR2和StGAME4是马铃薯块茎SGA生物合成的关键基因。

The content of SGAs(Steroidal glycoalkaloids)was evaluated in the leaf and peel tissue of 11 potato resources,the resources with relatively low level of SGAs were selected.The gene expression related to the SGAs biosynthesis was determined in Xiazhai 65,LZ111,Q072625,Longshu 7,Qingshu 9,and Qingshu 11,which would help confirm the key genes in SGAs biosynthesis of potato and provide the potential to create the low SGAs potato resources in the future.These results showed that SGAs content in the leaves of 11 cultivars was 0.08600–1.58066 mg·g^(-1)FW;the SGAs content in the peel tissue of potato tubers was 0.01415–0.18450 mg·g^(-1)FW and lower than 0.20 mg·g^(-1)FW(the safety level).The genes of StSQS1,StCAS,StSSR2 and StGAME4 played key roles in SGAs biosynthesis.