清解益肺苗方颗粒薄层鉴别方法研究

Study on the Thin Layer Ldentification Method of Qingjieyi Feimiao Formula Granule

目的:建立清解益肺苗方颗粒的薄层色谱鉴别方法。方法:以黄芪、南沙参、余甘子、鱼腥草、白花蛇舌草为对照,以三氯甲烷-甲醇(10∶1)、三氯甲烷一甲醇一甲酸(9∶1∶0.5)、石油醚(60~90℃)-乙酸乙酯-甲酸(5.5∶4.5∶0.25)、甲苯-乙酸乙酯-甲酸(9∶3∶0.5)、甲苯-丙酮-冰醋酸(8∶3∶0.3)为展开剂分别对颗粒中的主药黄芪、南沙参、余甘子、鱼腥草、白花蛇舌草进行薄层鉴别方法的研究。结果:薄层色谱斑点清晰,重现性好,专属性好,阴性无干扰。结论:建立的五味中药的薄层鉴别方法可作为清解益肺苗方颗粒医院制剂质量标准的参考依据。

Objective To establish a TLC identification method for Qingjieyifei Miao Fang granules.Methods Astragalus membranaceus, Radix Ginseng, Fructus embaldii, Herba houttuyniae and Hedyotis diffusa were used as control, chloromethanol-methanol(10: 1), chloromethanol-formic acid(9∶1∶0. 5), petroleum ether(60-90 ℃)-ethyl acetate-formic acid(5. 5∶4. 5∶0. 25),toluene-ethyl acetate-formic acid(9∶3∶ 0. 5), toluene-acetone-acetic acid(8∶ 3∶ 0. 3) were used as the developing agents to study the TLC identification methods of astragalus membranaceus, Radix Ginseng, Fructus polyphylla, Herba houttuyniae and Hedyotis diffusa in the granules.Results TLC spots were clear, reproducible, specific and negative without interference.Conclusion The TLC identification method of five Traditional Chinese medicines can be used as a reference for the quality standard of Qingjie Yifei Miao Fang granules.