藏药柳兰中黄酮类成分研究

Study on Flavonoids from Zang Medicine Epilobium angustifolium L.

目的:研究藏药材柳兰(Epilobium angustifolium)中黄酮类化学成分。方法:采用正、反相硅胶、MCI大孔树脂、Sephadex LH-20凝胶柱色谱及中低压柱色谱、制备型高效液相色谱分离纯化化合物,利用核磁共振和质谱等方法确定化合物结构。结果:从植物柳兰全草乙醇提取物的乙酸乙酯萃取部位中分离得到11个化合物,分别鉴定为槲皮素-3-O-β-D-葡萄糖醛酸苷(1)、金丝桃苷(2)、槲皮苷(3)、木犀草素(4)、槲皮素(5)、阿福豆苷(6)、山奈酚-3-O-α-L-阿拉伯呋喃糖苷(7)、槲皮素-3-O-α-L-阿拉伯呋喃糖苷(8)、桑色素-3-O-α-L-鼠李糖苷(9)、槲皮素-3-O-α-D-阿拉伯吡喃糖苷(10)和芦丁(11)。结论:从藏药材柳兰乙醇提取物的乙酸乙酯萃取部位中分离得到11种黄酮类成分。其中,化合物1为首次从柳兰中分离得到,化合物7、9、10为首次从柳叶菜科植物中分离得到。

Objective To investigate the chemical constituents of flavonoids from Zang medicine Epilobium angustifolium L..Methods The silica gel, reversed phase C-18 silica gel, MCI-gel, Sephadex LH-20 column chromatographic technique and preparative high performance liquid chromatography were applied to isolate and purify the chemical constituents from Epilobium angustifolium L..The structures of the isolated compounds were identified by means of MS and NMR spectroscopy.Results 11 compounds were isolated from the ethanol extracts of Epilobium angustifolium L., which were identified as quercetin 3-O-β-D-glucuronide(1),hyperoside(2),quercitrin(3),luteolin(4),quercetin(5),afzelin(6),kaempferol-3-α-L-arabinofuranoside(7),quercetin-3-α-L-arabinofuranoside(8),morin-3-O-α-L-rhamnoside(9),quercetin-3-O-α-D-arabinopyranoside(10),and rutin(11).Conclusion Compounds 1 was isolated from this plant for the first time.Compounds 7,9 and 10 were isolated from Onagraceae for the first time.