洋金花与木本曼陀罗叶绿体基因组特征与系统进化分析

Characterization and phylogenetic analysis of complete chloroplast genome of Datura metel and Brugmansia arborea

目的以洋金花Datura metel和木本曼陀罗Brugmansia arborea为材料,分析其叶绿体基因组结构和特征,基于叶绿体组数据探讨洋金花和木本曼陀罗及茄科其他物种的系统发育关系。方法利用华大MGISEQ-2000PE150测序平台,双末端测序策略对基因组DNA建库测序,用NOVOPlasty组装叶绿体基因组,采用最大似然法(maximum likelihood,ML)构建系统进化树。结果洋金花和木本曼陀罗的叶绿体基因组全长为155934bp和155939bp,分别包含131和130个基因,GC值32.3%,具有典型的四分区域结构,包括1个大单拷贝区(large single copy,LSC)、1对反向重复区(inverted repeats,IR)和1个小单拷贝区(small single copy,SSC),各区域序列长度分别为86354、86278、25609、25720、18362、18221 bp。系统发育分析表明,洋金花与曼陀罗属的曼陀罗构成1单系分支,具有100%支持率,而木本曼陀罗属与曼陀罗属构成单系分支,具有100%支持率。结论结果支持洋金花隶属于曼陀罗属,与曼陀罗亲缘关系较近,木本的木曼陀罗属从曼陀罗属分出,独立为一个属。洋金花和木本曼陀罗叶绿体基因组信息为后期分子鉴定和群体遗传研究奠定基础。

Objective To analyze the structure and feature of complete chloroplast genome of medicinal plants Datura metel and Brugmansia arborea,so as to construct the phylogenetic relationship between the two species and their relatives.Methods MGISEQ-2000PE150 was used to sequence the geomic DNA of D.metel and B.arborea with the paired-end strategy in Beijing Genomics Institute(China).The complete chloroplast genome was assembled using NOVO Plasty software,and sequence analysis was performed based on gene annotation results.Phylogenetic analyses were performed using Maximum-Likelihood(ML)methods.Results The complete chloroplast genome of D.metel and B.arborea were 155934 bp and 155939 bp in length including 131 and 130 genes respectively,with a GC content of 32.3%.The chloroplast genome exhibited a typical quadripartite structure,including a large single copy region(LSC),a pair of inverted repeats(IR),and a small single copy(SSC),and the each region lengths of which were 86354,86278,25609,25720,18362,18221 bp,respectively.Phylogenetic analyses result indicated that D.metel was sister to D.stramonium with bootstrap 100%.The genera Datura and Brugmansia formed one monophyletic group with bootstrap 100%.Conclusion This study verified that D.metel belonged to the Datura,and it was related to D.stramonium.The genus Brugmansia should be separated from genus Datura.The information of chloroplast genome provided foundation for subsequent studies on molecular identification and genetic diversity.