针刺次髎对大鼠自主神经系统相关神经元放电频率的影响

The Influence of Acupuncture at Ciliao on Discharge Frequency of Autonomic Nervous System Related Neurons in Rats

目的:观察针刺次髎穴对脂多糖(lipopolysaccharide, LPS)致全身重度炎症模型大鼠血清炎症因子的影响及针刺次髎穴对正常大鼠孤束核(nucleus tractus solitarii, NTS)神经元、蓝斑(locus caeruleus, LC)神经元与迷走神经放电频率的影响。方法:将18只体质量230~250 g雄性SD大鼠随机分为正常组、模型组、次髎组,除正常组外,其余组大鼠腹腔注射6 mg·kg-1LPS制备全身重度炎症大鼠模型,次髎组分别于造模前后针刺次髎穴30 min, ELISA检测血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)及IL-6的水平。将30只体质量300~320 g的雄性SD大鼠随机分为孤束核组、蓝斑组、迷走神经组,所有大鼠均选取左侧次髎穴进行直刺,刺激30s,利用电生理技术记录孤束核、蓝斑、迷走神经神经元细胞放电频率,并采用滂胺天蓝标记所记录神经元的位置。结果:与正常组比较,模型组大鼠血清TNF-α、IL-1β、IL-6水平增加;与模型组比较,次髎组大鼠血清TNF-α、IL-1β、IL-6水平降低,差异均具有统计学意义(P<0.01)。与针刺前比较,针刺次髎可增加孤束核、蓝斑及迷走神经神经元放电频率,差异均具有统计学意义(P<0.05)。结论:针刺次髎穴可降低LPS模型大鼠血清炎性因子的水平,增强自主神经系统相关神经元的放电频率。

Objective: To observe the influence of acupuncture at Ciliao(BL 32) on serum inflammatory factors in rats with severe systemic inflammation induced by lipopolysaccharide(LPS),and on the firing frequency of neurons in nucleus tractus solitarii(NTS),locus coeruleus(LC) and vagus nerve in normal rats.Methods: A total of 18 male SD rats with a body mass of 230-250 g were randomly divided into the normal group, the model group and the Ciliao group.Except the normal group, the rats in the other groups were intraperitoneally injected with 6 mg·kg-1of LPS to prepare the rat model of severe systemic inflammation.The rats in the Ciliao group were respectively acupunctured at Ciliao for 30 minutes before and after modeling, and the levels of serum tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and IL-6 were detected by ELISA.Thirty male SD rats with a body mass of 300-320 g were randomly divided into the NTS group, the LC group, and the vagus nerve group.All the rats selected Ciliao on the left side for direct puncture, were stimulated for 30 seconds;the discharge frequency of NTS,LC and vagus nerve neurons were recorded by using electrophysiological techniques, and the location of the recorded neurons were marked with panidine sky blue.Results: Compared with the normal group, the serum TNF-α,IL-1 β and IL-6 levels of the model group increased.Compared with the model group, the serum TNF-α,IL-1 β and IL-6 levels in the Ciliao group decreased, and all the differences were statistically significant(P<0.01).Compared with before acupuncture, Ciliao acupuncture could increase the firing frequency of NTS,LC and vagus nerve neurons, and all the differences were statistically significant(P<0.05).Conclusion: Acupuncture at Ciliao point can reduce the level of serum inflammatory factors in LPS model rats, and enhance the firing frequency of autonomic nervous system related neurons.