长链非编码RNA BC015134及其下游靶基因在胃癌中的表达及临床意义

Expression of long non-woven RNA BC015134 and its downstream target genes in gastric cancer and its clinical significance

目的:探讨长链非编码RNA BC015134及其下游靶基因在胃癌中的表达及临床意义。方法:回顾性选取2018年1月—2019年1月在上海交通大学医学院附属瑞金医院卢湾分院接受胃癌根治术治疗的50例患者的癌组织和癌旁组织作为研究对象。RT-PCR检测BC015134和MMP11在胃癌组织和癌旁组织中的表达,评价其特异性,分析BC015134和MMP11表达与胃癌临床病理特征的关系,Spearman分析BC015134和MMP11的相关性,采用ROC曲线分析BC015134和MMP11在胃癌中的诊断价值。结果:检测BC015134和MMP11在胃癌组织中的表达程度,采用RT-PCR方法特异性较高,熔解曲线单峰特异;胃癌组织中BC015134的相对表达明显低于癌旁组织(P<0.001),MMP11的相对表达显著高于癌旁组织(P<0.001);BC015134和MMP11的表达水平与TNM分期、分化程度、肿瘤浸润程度、远处转移和淋巴转移密切相关(P<0.05),而与年龄、性别和肿瘤大小无相关性(P>0.05);BC015134的表达水平和MMP11的表达呈负相关(r=-0.367,P<0.05);胃癌组织中BC015134水平诊断胃癌的ROC曲线下面积为0.801(95%CI 0.721~0.890,P<0.001),胃癌组织中MMP11水平诊断胃癌的ROC曲线下面积为0.713(95%CI 0.613~0.821,P<0.001),胃癌组织中BC015134和MMP11联合诊断胃癌的ROC曲线下面积为0.868(95%CI 0.798~0.934,P<0.001),胃癌组织中BC015134和MMP11联合诊断胃癌的ROC曲线下面积明显优于BC015134和MMP11单独诊断,差异有统计学意义(均P<0.05)。结论:BC015134在胃癌组织中的表达降低,并与其下游靶基因MMP11的表达呈负相关;BC015134表达升高的患者更容易发生远处转移和淋巴转移,TNM分期、分化程度、肿瘤浸润程度更严重,联合检测BC015134和MMP11对胃癌有较高的诊断价值。

Objective:To investigate the expression of long-chain non-woven RNA BC015134 and its downstream target genes in gastric cancer and its clinical significance.Methods:Retrospectively 50 pairs of cancer tissues and adjacent tissues from patients who underwent radical gastrectomy in Luwan Branch of Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine from January 2018 to January 2019 were selected as the research objects.Real-time PCR detected the expression of BC015134 and matrix metalloproteinase 11(MMP11)in gastric cancer and adjacent tissues,evaluated its specificity,and analyzed it The relationship between the expression of BC015134 and MMP11 the clinicopathological characteristics of gastric cancer,spearman analyzed the correlation between BC015134 and MMP11,and the ROC curve was used to analyze the diagnostic value of BC015134 and MMP11 in gastric cancer patients.Results:The expression of BC015134 and MMP11 in gastric cancer was detected.The real time PCR method had high specificity and single peak specificity of fusion curve;the relative expression of BC015134 in gastric cancer was significantly lower than that in adjacent tissues(P<0.001),and the relative expression of MMP11 in gastric cancer was significantly higher than that in adjacent tissues(P<0.001);BC015134 and MMP11 expression The level is closely related to TNM stage,degree of differentiation,degree of tumor invasion,distant metastasis and lymphatic metastasis(P<0.05),but not related to age,gender and tumor size(P>0.05);the expression level of bc015134 was negatively correlated with the expression of MMP11(r=-0.367,P<0.05);the ROC curve area of BC015134 in gastric cancer tissue for diagnosis of gastric cancer was 0.801(95%CI 0.721-0.890,P<0.001),the ROC curve area of MMP11 in gastric cancer tissue for diagnosis of gastric cancer was 0.713(95%CI 0.613-0.821,P<0.001),and the ROC curve area of BC015134 and MMP11 in gastric cancer tissue for joint diagnosis of gastric cancer was 0.868(95%CI 0.798-0.934,P<0.001).The ROC curve area of BC015134 and MMP11 in the diagnosis of gastric cancer was significantly better than that of BC015134 and MMP11 alone(P<0.05).Conclusion:BC015134 The expression was reduced in gastric cancer tissues,and was negatively correlated with its downstream target gene MMP11.In clinicopathological features,it was found that patients with increased expression of BC015134 were more likely to have distant metastasis and lymphatic metastasis,and the TNM stage,degree of differentiation and tumor infiltration were more serious.The combined detection of BC015134 and MMP11 had high diagnostic value for gastric cancer.